摘要
毛茛科的罂粟莲花(Anemoclema glaucifolium)是中国西南部金沙江河谷地带特有的单种属植物。本研究利用分子生物学手段(SNPs),对罂粟莲花的遗传多样性和遗传结构进行了研究。三个叶绿体片段(rps16内含子,psbA-trnH基因间隔区以及trnC-ycf6基因间隔区)联合分析的结果显示低的遗传多样性和高的遗传分化。这可能是由于居群间长期的地理隔离,狭小的分布区以及生境片段化造成的有限的基因流所引起的。如今在金沙江上修建水电站的位置与罂粟莲花的分布区有部分重叠,这些水电站会淹没罂粟莲花的部分个体和生境,并且改变生态环境,威胁罂粟莲花的生存。本研究对罂粟莲花遗传多样性的研究为制定有效的保护策略提供了信息。
Anemoclema W.T. Wang, a monotypic genus of Ranunculaceae, is endemic to the Jinsha River Valley in southwest China. Combining field investigation with molecular marker analysis （SNPs） , we investigated the genetic diversity and genetic structure of A. glaucifolium. Analysis of three chloroplast DNA （cpDNA） regions （rpsl6 in- tron, psbA-trnH intergenic spacer and trnC-ycf6 intergenic spacer） revealed a low level of genetic diversity within the species, but high divergence among populations. This genetic structure is possibly caused by a long period of historical geographic isolation, a relatively narrow distribution range and limited gene flow due to habitat fragmenta- tion. Hydropower stations are scheduled to be built in the Jinsha River drainage system in areas that overlap the range of A. glaucifolium and as a result of their construction the habitats of A. glaucifolium will be flooded or adverse- ly affected in other ways, thus threatening the survival of the species. The results of our analysis of genetic diversity in A. glaucifolium are of value for developing an appropriate conservation strategy for this vulnerable species.
出处
《植物分类与资源学报》
CAS
CSCD
北大核心
2013年第5期555-562,共8页
Plant Diversity
基金
supported by grants from the National Natural Science Foundation of China(30870242)
