摘要
目的:初步探讨黄花倒水莲提取物对小鼠辐射损伤的防护作用。方法:以辐射损伤小鼠作为研究对象,分别以黄花倒水莲提取物2,4,8 g·kg-1·d-1灌胃,阴性对照组、辐射模型组给予等量生理盐水。灌胃5 d后,除阴性对照组外各组小鼠接受6 Gy的60Coγ射线一次性全身均匀照射,然后再继续灌胃10 d,最后1次灌胃24 h后,检测各组小鼠外周血中红细胞数(RBC)、白细胞数(WBC)、血小板数(PLT)、淋巴细胞数(Lymph),计算脾指数和胸腺指数,用单细胞凝胶电泳技术检测小鼠脾细胞、骨髓细胞的DNA损伤。结果:与阴性对照组比,辐射模型组、黄花倒水莲各剂量组小鼠的RBC数、WBC数、PLT数、Lymph数、胸腺指数、脾指数明显减少(P<0.01或P<0.05),脾细胞、骨髓细胞的尾部DNA百分率(Tail DNA%)和尾矩(Tail Moment)明显增大(P<0.01或P<0.05);但与辐射模型组比,黄花倒水莲各剂量组小鼠的RBC数、WBC数、PLT数、Lymph数、胸腺指数、脾指数明显增加(P<0.01或P<0.05),脾细胞、骨髓细胞的Tail DNA%和Tail Moment明显减少(P<0.01),存在剂量-反应关系。结论:黄花倒水莲提取物对辐射所致小鼠造血系统和免疫器官损伤有一定的防护作用。
Objective： To discuss protective effect of polygala fallax in radiation injured mice. Method： Polygala fallax extract was given at dose of 2, 4, 8 g·kg^-1·d^-1 respectively, negative control group add equivalent normal saline as control group. After 5 days of administration, in addition to the negative control group. micereceived disposable irradiate 6 Gy of ^60Co γ radiation uniformly, administration lasted 10 days. 24 h after end administration, red blood cell count （RBC）, white blood cell count （WBC）, platelet count （PLT）, lymphocytes （Lymph） , spleen index and thymus index, DNA damage of spleen cells and marrow cells were observed. Result： Compared with the negative control group, P. fallax decreased RBC, WBC, PLT, lymph thymus index, spleen index （P 〈 0.01 or P 〈 0.05） , and increased spleen ceils in bone marrow cells percentage of tail DNA and tail moment significantly （P 〈 0.01 or P 〈 0.05）. But compared with the control group, P. fallax increased RBC count WBC PLT, Lymph thymus index, spleen index significantly （P 〈0.01 or P 〈0.05） , and decreased spleen cells in bone marrow ceils percentage of tail DNA and Tail Moment significantly （P 〈 0.01 ）. There is dose- response relationship..Conclusion： P. fallax extract have certain protective effect on hemopoietic system and immune organs in mice caused by radiation injury.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第19期234-237,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西教育厅2011年科研基金项目(200103YB121)
玉林师范学院高层次人才科研启动基金项目(G20120017)
