摘要
对在生物学特性特别是寄主范围上存在明显差异的 NSP株系和 NS株系的代表分离物 (广州天河分离物和高州分离物 )的 DNA组分 1进行了克隆和序列分析 ,结果表明 :2个代表分离物的DNA组分 1全序列、ORF及其编码的氨基酸序列的变异率分别为 3.2 %、3.1%和 2 .8% ,从而进一步确证了可以用寄主范围来作上述 2个株系的鉴定。此外 ,这 2个株系的 DNA组分 1、ORF及其编码的氨基酸序列分别与肖火根等报道的中国 (广东 )分离物、以及亚洲组和南太平洋组各分离物进行比较 ,结果表明 :NS株系与肖火根等报道的中国 (广东 )分离物的亲缘关系很密切 ;这 2个株系与亚洲组各分离物的差异均较小 ,而与南太平洋组各分离物的差异均较大 。
The two Guangdong BBTV representative isolates (Guangzhou Tienhe isolate and Gaozhou isolate) were found to have different biological characteristics, especially on host range, so that they were considered as two different strains. In order to prove it, the DNA component 1 of the two isolates were respectively cloned and sequenced. The results showed that the variation ratios of full length DNA component 1, ORF and amino acid sequence between the two isolates were 3.2%, 3.1% and 2.8% respectively. In addition, DNA component 1,ORF and encoding amino acid sequence of the two strains were compared with those of Xiao's Chinese (Guangdong) isolate, the isolates of the Asian group, and the isolates of the South Pacific group. The results showed that the Xiao's isolate are more closely related to the NS isolate than to the NSP strain, that both the NSP strain and the NS strain are more closely related to the isolates of the Asian group than to the isolates of the South Pacific group. They should belong to the Asian group.
出处
《植物病理学报》
CAS
CSCD
北大核心
2000年第4期364-369,共6页
Acta Phytopathologica Sinica
基金
国家自然科学基金(39870434)
高等学校博士点基金(950503)
广东省自然科学基金资助项目(980126)
关键词
香蕉束顶病毒
株系
基因克隆
序列分析
Banana bunchy top virus (BBTV)
strain
gene cloning
sequencing
