摘要
目的探讨miR-33b基因上游CpG岛甲基化对该基因在胃癌组织中表达情况的影响,分析其表达与临床统计学资料间的关系。方法使用Taqman real-time PCR法检测miR-33b在42例胃癌患者癌及癌旁组织中的相对表达情况,并分析miR-33b表达水平与胃癌临床病理特征间的关系,随后使用组织基因组DNA快速提取试剂盒提取胃癌组织及正常人血液基因组DNA,经重亚硫酸氢盐处理后,甲基化特异性PCR法检测其上游CpG岛的甲基化程度,PCR产物经琼脂糖凝胶检测,并进行统计分析。结果 miR-33b表达下调与胃癌患者的年龄、性别、发生部位、有无脉管转移、肿瘤分型无相关性,但与肿瘤是否发生远处转移具有相关性(P<0.05),且其低表达与上游CpG岛的甲基化沉默修饰密切相关(P<0.05)。结论 miR-33b的表达在胃癌中受甲基化调控且与胃癌远处转移相关。
Objective To investigate the impacts of miR-33b down-regulation by DNA Methylation in gastric cancer （GC） and the association between miR-33b level and various clinieopathological factors. Methods Expression of miR-33b was detected by Taqman real-time PCR in 42 paired GC samples. The association between miR-33b level and various clinicopathological factors was analyzed. Genomic DNA samples were amplificated by PCR after modifica- tion by sodium bisulfite using the EpiTect Bisulfite Kit then the methylation degree of CpG island upstream of miR- 33b gene was detected by methylation specific PCR （MSP）. Results The lower level expression of miR-33b was not associated with gender, age, venous invasion, position, borrmann typing, pT stage, pN stage, but significantly asso- ciated with gastric cancer metastasis （ P 〈 0. 05） and regulated by CpG island hypermethylation （ P 〈 0. 05）. Conclu- sions miR-33b may be regulated by DNA methylation and act as a tumor suppressor in gastric cancer.
出处
《基础医学与临床》
CSCD
北大核心
2013年第10期1269-1274,共6页
Basic and Clinical Medicine
基金
国家自然科学基金(91129716)
