摘要
目的 探讨抗氧化剂N -乙酰半胱氨酸 (NAC)对急慢性吸烟大鼠气道炎症的影响。方法 将Wistar大鼠随机分成 :①吸烟组 ,②干预 (吸烟 +NAC)组 ,③对照组。其中吸烟组和干预组中动物均动态观察第 1,2 ,7,14 ,6 0天的气道炎症反应 ,包括肺组织病理变化 ,肺泡灌洗液 (BALF)中细胞计数和分类 ,同时测定BALF中IL -8含量。结果 急慢性吸烟大鼠的支气管周围和肺间质均有大量炎症细胞浸润 ,BALF中白细胞总数和中性粒细胞数均显著高于对照组 ;其中以急性吸烟早期 (第 1,2天 )炎症细胞浸润明显和细胞数的升高最大 ,慢性吸烟 (6 0d)炎症细胞浸润和细胞数的升高有所减少。而BALF中IL -8水平 ,在吸烟至第 7,14 ,6 0天才显著高于对照组。NAC能减少炎症细胞浸润 ,并显著降低BALF中白细胞总数、中性粒细胞数和IL -8水平。结论 急慢性吸烟在大鼠气道炎症反应中起一定的作用 ;
Objective To assess the effect of N-acetylcysteine (NAC, antioxidant) on the airway inflammation in rats with acute and chronic smoking. Methods\ All Wistar rats were divided randomly into three groups: ①smoking group, ②intervention group,③control group. The former two groups were to observe the indexes of airway inflammation during different periods (1, 2, 7, 14, 60 d) including pathologic changes of lung tissues, the counting and classifying of white blood cells(WBC) and the concentration of IL-8 in bronchial alveolar lavage fluid(BALF). Results\ For the acute and chronic smoking rats, the infiltrating inflammation cells were found around bronchiolus and in the lung interstium, Total WBC and neutrophils in BALF were more than those in control group, peaking at early stage (acute smoking d 1, 2), and then decreasing (chronic smoking 60 d); but the concentration of IL-8 in smoking group on 7, 14, 60 d were higher than that in control, NAC could inhibit the infiltrating of inflammation cells and significantly decreased total WBC and neutrophils and the concentration of IL-8 in BALF. Conclusion\ Acute and chronic smoking have a role in rats airway inflammation reaction which can be inhibited by NAC.
出处
《广东医学》
CAS
CSCD
2000年第11期913-915,共3页
Guangdong Medical Journal
