摘要
石参为百合科独尾草属独尾草(Eremurus chinensis Fedtsch.)肉质根加工的稀有山野菜,主产于秦岭南坡局地。通过单因素和正交试验,研究了苯酚-硫酸法测定石参多糖含量时各因素对结果的影响,并对这些因素进行了优化和方法学验证。研究结果表明,苯酚用量1.6 mL、浓硫酸用量7.0 mL、显色时间30 min、显色温度80℃为苯酚-硫酸法测定石参多糖的最佳工艺条件,在此条件下平均回收率为98.64%,相对标准偏差(RSD)为1.40%;优化后的苯酚-硫酸法测定石参多糖准确性和稳定性高。比较分析表明,不同植物多糖的苯酚-硫酸法检测工艺有一定的差异,浓硫酸的加入方式对测定结果有较大影响。
Shishen, a rare edible wild herb produced in Qinling Mountains, is the process fleshy roots from Eremurus chinensis belonging to genus of Eremurus, Liliaceae. Through the single factor experiments and orthogonal experiments, the factors affecting the determination of polysaccharide content in Shishen （E. chinensis） with phenol-sulfuric acid method were optimized. The results showed that the optimum determina- tion conditions were 1.6 mL phenol, 7.0 mL sulphuric acid, 80 ℃ reaction temperature and 30 min reaction time. The average recovery was up to 98.64%, and the RSD was 1.40%. The comparative analysis indicated that the way used with phenol-sulfuric acid and how to add sulphuric acid maybe result in a great of influence to the results for different polysaccharides test. In addition, the modified phenol-sulfuric acid method to deter- minate polysaccharides is accurate and steady.
出处
《陕西理工学院学报（自然科学版）》
2013年第5期64-70,共7页
Journal of Shananxi University of Technology:Natural Science Edition
基金
陕西省教育厅科研计划项目(12JS026)
关键词
石参
多糖
苯酚
硫酸法
条件优化
Shishen（Eremurus chinensis）
polysaccharide
phenol-sulfuric acid method
processoptimization
