摘要
目的 探讨He Ne激光重复照射对培养增生性瘢痕成纤维细胞胶原合成的抑制作用。方法 以不同功率密度 (10、5 0、10 0和 15 0mW /cm2 )He Ne激光照射培养增生性瘢痕成纤维细胞 30min ,1/d ,连续照射 3d ,在 3d重复照射后 2 4h用3H 脯氨酸掺入法和斑点杂交法分别检测成纤维细胞胶原合成和I型前胶原基因表达。结果 培养增生性瘢痕成纤维细胞的胶原合成、I型前胶原基因表达水平在 10、5 0mW /cm2 照射后无变化 ,10 0mW /cm2 重复照射后降低 ,与对照组相比有显著性差异 (P <0 .0 5 ) ;以 15 0mW /cm2 重复照射 ,细胞胶原合成与I型前胶原基因表达水平亦显著低于对照组 (P <0 .0 5 ) ,且 15 0mW /cm2 照射后的I型前胶原基因表达水平低于 10 0mW /cm2 照射 (P <0 .0 5 )。结论 10 0mW /cm2 或 15 0mW /cm2 功率密度He Ne激光重复照射能抑制培养增生性瘢痕成纤维细胞胶原合成 。
Objective To explore the inhibitory effects of He Ne laser repeated irradiation on the collagen synthesis of cultured scar fibroblasts. Methods Cultured fibroblasts derived from hypertrophic scars (HS) were irradiated with He Ne laser for 30 min at various power densities (10 mW/cm 2, 50 mW/cm 2, 100 mW/cm 2 and 150 mW/cm 2), once a day for 3 consecutive days. In 24 h after repeated irradiation collagen production and type I procollagen mRNA level of fibroblasts were measured with the incorporation of [ 3H] proline and blot hybridization techniques respectively. Results Collagen synthesis and type I procollagen mRNA level remained unchanged when the laser was irradiated at the power density of 10 mW/cm 2 or 50 mW/cm 2. Compared with control, collagen synthesis and type I procollagen mRNA level were significantly decreased at the power density of 100 mW/cm 2 or 150 mW/cm 2 ( P <0.05). Type I procollagen mRNA level at the power density of 150 mW/cm 2 was lower than that at 100 mW/cm 2 ( P < 0.05) . Conclusion Repeated He Ne laser irradiation at the power density of 100 mW/cm 2 or 150 mW/cm 2 can suppress collagen synthesis of cultured fibroblasts in HS. The cause of suppression may be associated with down regulation of type I procollagen mRNA expression.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2000年第11期1094-1096,共3页
Journal of Third Military Medical University
关键词
HE-NE激光
增生性瘢痕
成纤维细胞
胶原合成
He Ne Laser
hypertrophic scar
fibroblast
collagen synthesis
type I procollagen mRNA
