摘要
目的 研究低硒 (Se)低维生素 E(VE)能否诱导大鼠肝细胞凋亡及 c AMP和细胞内 Ca2 + 所起的作用。方法 以天然的和人工半合成的低 Se低 VE饲料喂养大鼠 17周 ,采用流式细胞术检测肝细胞凋亡 ,采用放免法检测 c AMP含量 ,Fura- 2负载荧光分光光度法测定细胞内 Ca2 + 含量。结果 与补 Se和 VE组大鼠相比 ,低Se低 VE组大鼠肝细胞凋亡显著增加 ;c AMP含量明显升高 ;细胞内 Ca2 + 含量明显升高。结论 微量营养素 Se和 VE缺乏可诱导大鼠肝细胞凋亡 ;c AMP和细胞内 Ca2 +
Objective To study whether dietary deficiency of selenium (Se) and vitamine E (VE) can induce hepatocyte apoptosis and what is the role of cAMP and Ca 2+ in the apoptotic process.Methods The experiment was performed on rats kept on natural or semisynthetic diet deficient in Se and VE for 17 weeks.The hepatocyte apoptosis was detected by using flow cytometry in rats.cAMP was detected by using radioimmunoassay,intracellular free Ca 2+ was assayed by fluorescence spectrophotometry after the hepatocytes were loaded with a fluorescence probe (Fura 2AM).Results As compared with Se and VE supplemented groups,hepatocyte apoptosis was obviously increased in rats kept on Se and VE deficient diet,accompanied with an increase of cAMP and Ca 2+ .Conclusions The dietary deficiency of Se and VE can induce hepatocyte apoptosis in rats;cAMP and Ca 2+ may be involved in the regulation of the apoptotic process.
出处
《中国地方病学杂志》
CAS
CSCD
2000年第6期423-425,共3页
Chinese Jouranl of Endemiology
基金
国家"八五"科技攻关专题资助!(85 917-0 1-0 1)
