摘要
桉树青枯病是由Ralstonia solanacearum引起的一类土传病害，具有发病快速不易防治等特点。为了实现桉树组培苗和轻基质带菌情况的早期快速检测，将不同浓度青枯菌悬液人工接种于桉树组培苗和轻基质中，利用特异性寡核苷酸引物OLI1／Y2，能快速的检测出潜伏期组培苗，以及带菌轻基质中青枯菌的数量。试剂盒法提取基因组DNA后对其灵敏度检测结果表明：试剂盒法提取基因组DNA纯度较高，通过微量分光光度计检测出A260／A280均在1．7～1．9之间。桉树组培苗组织和轻基质中检测限分别为10^2CFU／mL和10^3CFU／mL。因此，潜伏期PCR快速检测法为预防病原菌的进一步传播提供了新的思路。
Bacterial wilt is rapid onset and not easy to control, and is a kind of soilborne pathogen caused by Rabtonia solanacearum. In order to realize rapid detection of the pathogens from tissue culture seedling and substrates as early as possible in latent infection, a pair of primers OLII/Y2 was used by amplify 16S rRNA sequence to rapidly detect the pathogens and the numbers in Ralstonia solanacearum. Then, genomic DNA was extracted by using kit. The results show that the purity values of DNAA260/A280 were in the 1.7 1.9; the detection limits of Eucalyptus tissue culture organization and substrates were 102 CFU/mL and 1 03 CFU/mL respectively. Therefore, the fast-detection method can provide a new way to prevent and control dissemination of bacterial wilt.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2013年第9期42-45,共4页
Journal of Central South University of Forestry & Technology
基金
拮抗青枯病菌菌株的筛选及应用技术(2012BAD19B08)
桉树重大病虫害控制技术研究与示范(2010kjcx015-03)
关键词
桉树青枯病
检测灵敏度
组培苗
轻基质
聚合酶链反应法
eucalyptus bacterial wilt
detection sensitivity
tissue culture seedling
light media
polymerase chain reaction method
