摘要
目的探讨熊果酸(UA)抑制人急性髓性白血病细胞系(HL-60)细胞增殖和诱导凋亡作用,并观察Bcl-2和bax基因表达的影响。方法体外培养HL-60细胞。MTT比色法检测增殖活性;流式细胞术(FCM)检测细胞凋亡和细胞周期分布;Western Blot分析Bcl-2和bax基因表达。结果 UA显著抑制HL-60细胞增殖,呈浓度依赖性;UA呈浓度依赖性诱导HL-60细胞凋亡,明显阻滞于细胞周期G1期;UA以浓度依赖方式下调Bcl-2蛋白表达和上调bax蛋白表达。结论 UA抑制HL-60细胞增殖和诱导凋亡,其作用机制与下调Bcl-2上调bax蛋白表达相关。
Objective To investigate the effect of UA on the apoptosis and expressions of Bcl-2/bax in HL-60 cells. Methods HL-60 cell line was cultured in vitro. MTT methods was used to detect the inhibitory effect of UA on proliferation. Flow cyetmetry was used to observe the effect of UA on the apoptosis and cycle distribution of HL- 60 cells. The influence of UA on the bcl-2 and bax protein expression of HL-60 cells was analyzed with western blot. Results UA markedly inhibited proliferation of HL-60 cells in a concentration-dependent manner, UA induced apoptosis of HL-60 cells in a concentration dependent manner,and decreased the proportion of cells in the G1 phase. UA can efficiently up-regulated bax and down-regulated bcl-2 protein expression of HL-60 ceils in a concentration dependent manner. Conclusion UA inhibited proliferation and induced apoptosis on HL-60 cells partly by up- regulating expression of bax and down-regulating expression of bcl-2.
出处
《中国医药科学》
2013年第17期30-32,共3页
China Medicine And Pharmacy
基金
湖南省教育厅青年项目资助(10B099)
