摘要
目的调查福建福州地区汉族群体遗传15个STR基因座多态性参数，同时评估AmpFlmSTR IdentifilerTM体系应用于该地区汉族人群进行法医学个体识别及亲子鉴定的价值。方法应用AmpFlmSTR IdentifilerTM体系荧光标记复合扩增系统检测350名福建福州地区汉族无关个体15个STR基因座的多态性，统计计算群体遗传学参数。结果15个STR基因座的基因型分布均符合Hardy—Weinberg平衡（P〉0．05），发现4个稀有等位基因。15个STR遗传标记均具有高度多态性，杂合度0．12～0．405，匹配概率0．032～0．225，个体识别力0．775～0．968，多态性信息含量0．55—0．86，非父排除率0．285～0．761。结论15个STR基因座在福建福州地区有较高的多态性。通过这份样本实验和等位基因频率总结，得到了福建福州人群更多法医学亲权鉴定和个人识别等位基因多样性的客观数据。
Objective To investigate the genetic polymorphism and its Forensic application of 15 STR loci from AmpFlmSTR IdentifilerTM system in personal identification and paternity testing in Han race population in Fuzhou Fujian. Methods Allele frequencies for 15 STR loci found in AmpFlmSTR IdentifilerTM kit were determined in a sample of 350 unrelated Chinese individuals in Fuzhou Fujian.Population genetics parameter for Forensic using were calculated. Results No deviations of the observed allele frequency from Hardy-Weinberg equilibrium expectations were found for Chi-square test （P 〉 0.05）. 4 off ladder alleles （at 15 STR loci） were found. All 15 loci in AmpFlmSTR IdentifilerTM system show highly polymorphic, Observed heterozygosity（Ho） varies between 0.12and 0.405, matching probability between 0.032 and 0.225, power of discrimination between 0.775and 0.968, polymorphic information content （PIC） varies between 0.55 and 0.86, power of exclution between 0.285 and 0.761. Conclusion All 15 loci in AmpFlmSTR IdentifilerTM system show highly polymorphic in Fuzhou Fujian Han race population. With the analysis of alleles in this sample and allele frequenciecy, objective alleles genetic polymorphism data about paterniety testing and personal identification have been obtained among fuzhou fujian Han race population.
出处
《中国医药科学》
2013年第17期14-16,32,共4页
China Medicine And Pharmacy
关键词
STR基因座
遗传多态性
等位基因频率
福州地区
Short tandem repeat （STR） loci
Genetic polymorphisms
Allele frequency
Fuzhou area
