摘要
目的:研究胃癌中SLC7A5转录和表达的特点,探讨SLC7A5对胃癌细胞增殖的影响及其在胃癌发生、发展中的临床意义。方法:应用免疫组化法检测52对临床采集的胃癌组织及相应的癌旁组织中SLC7A5的表达情况;分析4株胃癌细胞(SGC-7901、MKN-45、MGC-803和CRL-5974)中SLC7A5转录及表达水平与永生化胃黏膜细胞GES-1间的差异;结合临床病理指标,分析SLC7A5在胃癌组织中的表达水平与各项临床病理特征间的相关性;并应用pGU6/GFP/Neo/shRNA-SLC7A5质粒载体转染胃癌SGC-7901细胞,下调SLC7A5表达,通过CCK8检测及流式细胞术检测分析SLC7A5对胃癌细胞增殖和细胞周期的影响。结果:SLC7A5在胃癌组织和细胞中的表达上调,且其表达水平与胃癌肿瘤的直径、局部浸润深度、淋巴结转移及TNM分期进展显著相关;而下调胃癌SGC-7901细胞SLC7A5表达,可显著减弱胃癌细胞增殖能力,并将细胞周期阻滞于G0/G1期。结论:SLC7A5可促进胃癌细胞增殖,与其肿瘤直径、局部浸润深度、淋巴结转移及TNM分期等临床病理特征相关,可作为分子标志物评价胃癌的生物学行为,并可能成为胃癌诊断和防治中的新靶点。
Objective:To investigate the transcription and expression of SLC7A5 in GC tissue and cell lines,and analyze the influence of SLC7A5 on GC cell proliferation and its clinicopathologic significance.Methods:Levels of SLC7A5 expression in GC tissues and match paired non-cancerous tissues from 52 patients were detected by immnohistochemistry analysis.Differences in transcription and protein expression of SLC7A5 between GC cell lines(SGC-7901,MKN-45,MGC-803 and CRL-5974) and human gastric epithelial immortalized GES-1 cells were surveyed.Correlations between the expression of SLC7A5 and the clinicopathologic features of GC were analyzed.GC SGC-7901 cells,in which expression of SLC7A5 was down-regulated by pGU6/ GFP/Neo/sh-RNA,were studied for cell proliferation ability and cell cycle by applying CCK assay and flow cytometry.Results:The expression of SLC7A5 in GC tissues was up-regulated and associated significantly with tumor size,local invasion,lymph node metastasis and advanced TNM stage.Down-regulation of SLC7A5 expression in SGC-7901 cells inhibited the ability of cell proliferation,along with significant G0/G1 cell cycle arrest.Conclusions:SLC7A5 confers GC cells with greater proliferation and is associated with the clinicopathologic features of GC,including tumor size,local invasion,lymph node metastasis and TNM stage.SLC7A5 could be a biomarker for predicting GC biological behavior,and perhaps become a new target for GC diagnosis and treatment.
出处
《诊断学理论与实践》
2013年第3期284-289,共6页
Journal of Diagnostics Concepts & Practice
基金
国家自然科学基金(30900670
81272749)
上海交通大学医学院科技基金(12XJ10049)
关键词
胃癌
SLC7A5
细胞增殖
临床病理
Gastric cancer
SLC7A5
Cell proliferation
Clinicopathology
