摘要
目的：确定毛乳头细胞（DPC）对毛囊前体黑素细胞（MP）的激活和趋化作用。方法：采用三维分离式共培养体系，将MP与毛囊外毛根鞘角质形成细胞（KC）以1：10比例于6孔培养板中接触式共培养，DPC以1×10^6个／孔分离培养于Transwell inserts。检测DPC对MP的趋化作用。MP内黑素小体、酪氨酸酶（TYR）、酪氨酸酶相关蛋白1（TRP-1）和酪氨酸酶相关蛋白2（TRP-2）的表达。结果：MP与KC共培养后MP内出现Ⅲ期黑素小体，当三种细胞共培养后MP内主要为Ⅲ和Ⅳ期黑素小体。DPC可促进MP中TYR和TRP-1的表达，但对TRP-2的表达无明显影响，对MP具有明显趋化作用。结论：构建了MP、KC和DPC的三维培养体系，发现DPC对MP具有明显激活和趋化移行作用。
Objective： To assess the effects of dermal papillar cells （DPC） on activation and migration of melanocytes precursors （MP） . Methods： MP and kyratinocyte （KC） cultured from outer root sheath were seeded into 6-well dishes in the ratio of 1：10, and DPC were seeded separately into transwell inserts. Then the changes of MP morphology were observed and the changes of tyrosinase （TYR）, tyrosinase related protein -1 （TRP-1） and tyrosinase related protein-2 （TRP-2） before and after treatment with DPC. KC and MP were co-cultured in ratio of 1：10 in transwell inserts, and DPC were seeded into 6-well dishes in three differ- ent concentrations to detect the chemotaxis of DPC on MP. Results ： Electron microscopy showed that stage III melanosomes were found in MP after co-cultured with KC and the melanosomes were mainly in stage III and IV after three-dimensional cultured. DPC promoted the expression of TYR and TRP- 1 in MP, but had no sig- nificant effect on the expression of TRP-2. The DPC had an obvious chemotaxis on MP. Conclusions： DPC has a significant effect on the activation and chemotaxis migration of MP in the constructed three-dimensional culture system.
出处
《中国麻风皮肤病杂志》
2013年第9期557-560,共4页
China Journal of Leprosy and Skin Diseases
基金
国家自然科学基金资助项目(编号:81000703)
江苏省自然科学基金(编号:BK2009437)
2008杨森科学基金
关键词
毛囊
前体黑素细胞
毛乳头细胞
激活
移行
hair follicle
melanocyte precursors
dermal papillar cells
activation
migration
