摘要
目的探讨金标法和免疫发光法检测甲胎蛋白在健康体检中的应用。方法选取2010年10月至2012年10月期间来本院进行健康体检的患者1087例,全部给予金标法和免疫发光法进行甲胎蛋白检测,对两组方法的监测结果进行比较。结果金标法检测甲胎蛋白阳性10例,阴性1077例;免疫发光法检测甲胎蛋白阳性4例,阴性1083例。金标法检测甲胎蛋白的敏感性为100%,特异性为99.45%,准确性为99.45%。结论金标法在检测甲胎蛋白上具有较高的敏感性、特异性、准确性,并较免疫发光法更加方便、简便、快速,对健康体检筛查患者具有重要意义,目前本院在对健康人进行甲胎蛋白检测中,首先使用金标法进行定性检查,对于阳性结果病例再进行免疫发光法进行定量检查,这样可以大大降低临床检测成本,大大缩短检测时间,并且可以提高准确率有效降低假阳性病例的发生,值得临床推广使用。
Objective To investigate the application of gold standard method and immunoassay in detection of AFP in health examination. Methods 1087 patients from 2010 October to 2012 October to our hospital for health examination, all give the gold standard method and immunoassay for detection of alpha-fetoprotein, compared to two groups monitoring method. Results The gold standard method for the detection of alpha-fetoprotein positive in 10 cases, 1077 cases were negative; immunoassay in detection of AFP was positive in 4 cases, 1083 cases were negative. The sensitivity of the gold standard of alpha- fetoprotein detection me^od was 100%, the specificity was 99.45%, the accuracy was 99.45%1 Conclusion The gold standard method has the accuracy, specificity, high sensitivity in the detection of alpha-fetoprotein, and immune luminescence method is more convenient, simple, fast, has the vital significance to the health screening patients, the hospital in the detection of alpha-fetoprotein in healthy people, the first to use the gold standard method. Check,for positive results were then immunoluminometric assay for quantitative examination, which can greatly reduce the cost of clinical detection, greatly shortens detection time, and can improve the accuracy effectively reduce the false positive cases, worthy of clinical use.
出处
《中国医药指南》
2013年第24期34-35,共2页
Guide of China Medicine
关键词
金标法
免疫发光法
甲胎蛋白
健康体检
Gold standard method
Immunoassay
Alpha-fetoprotein
Health examination
