摘要
研究桑叶中总黄酮的酶解-超声波-表面活性剂协同提取的工艺条件。以总黄酮提取率为考察指标,对酶解温度、pH值、Tween-80质量浓度、提取时间进行单因素试验。在单因素试验基础上,利用正交试验优化总黄酮提取工艺条件。结果表明,最佳工艺参数为:酶解温度50℃,pH值6.5,Tween-80质量浓度1.00%,提取时间50min。酶解-超声波-表面活性剂协同提取是一种快速、高效的提取桑叶总黄酮方法。
Enzymatic hydrolysis-ultrasound-surfactant extraction technology of total flavones from mulbery leaves was studied. Single factor experi- ments were conducted in the effect of temperature of enzymatic hydrolysis, pH value, concentraction of Tween-80, extraction time. Based on the re- suits of single factor test, orthogonal test were employed to optimize the extraction conditions. The optimum extraction conditions of extracting total flavones from mulbery leaves was showned as followes： temperature of enzymatic hydrolysis 50℃, pH value 6.5, concentraction of Tween-80 solution 1.00%, extraction time 50rain, The enzymatic hydrolysis-ultrasound-surfactant extraction method was proved to be a fast, efficient extraction method.
出处
《中国酿造》
CAS
2013年第8期117-119,共3页
China Brewing
关键词
酶解
超声波
表面活性剂
桑叶
总黄酮
enzymatic hydrolysis
ultrasound
surfactant
mulbery leaves
total flavones
