摘要
目的探讨白细胞介泰．6受体（IL-6R）基因单核苷酸多态性（SNP）及单体型与2型糖尿病的相关性。方法选择入住本院的88例2型糖尿病（T2DM）患者为2型糖尿病组，以同期体检并糖耐量正常的98例健康自愿者为对照组，采用聚合酶链反应限制性片段长度多态性（PCR—RFLP）方法对IL-6R基因的2个SNP位点予以基因型检测。采用聚类分析方法分析基因多态性特征。结果2型糖尿病组D358A基因型中A等位基因频率较对照组高，C等位基因频率较对照组降低，差异有统计学意义（P〈0．05）。2型糖尿病组-183（G—A）基因型频率与等位基因频率和对照组比较，差异均无统计学意义（P〉0．05）。A2-G单体型在两组中最为常见，其频率分布为0．391、0．288（P=0．053）。在4种单体型中两组仅C—A．单体型的频率差异有统计学意义，分别为0．103、0．281（P=0．001）。结论IL-6R基因第9外显子D358A（rs8192284）是种有价值的遗传性标志，C等位基因对2型糖尿病具有保护作用，携带C—A：单体型的人群较易患2型糖尿病。
Objective To discuss interleukin-6 receptor（IL-6R） single nucleotide polymorphism （SNI＇） gene and haplotype with type 2 diabetes mellitus （T2DM）. Methods Enshi region Tujia, Miao in 88 patients with type 2 diabetes mellitus as group T2DM, selected from the same area in normal glucose tolerance in 98 healthy volunteers as a control group, respectively PCR-RFLP technology on IL-6R gene of 2 SNP loci to genotypes were detected. By using cluster analysis method for the analysis of gene polymor- phism. Results In group T2DM, D358A genotype frequency and allele frequencies were compared with control group, the difference was statistically significant （ P 〈 O. 05 ）. The T2DM in group-183 （ G---A ） genotype frequency and allele frequencies were compared with control group, the difference was not statis- tically significant（ P 〉 0.05 ）. A^-G in the two groups are the most common, the frequency distribution of O. 391/0. 288 （P = 0. 053 ）. In four kinds of haplotypes in two groups of only C-A2 frequency difference has statistics significance,respectively O. 103/0. 281 （ P = 0. 001 ）. Conclusion The IL-6R gene exon ninth D358A（rs8192284） is a kind of valuable genetic markers, C allele of T2DM play a protective role, carrying the C-A2 haplotypes in the population is susceptible to T2DM, so it is needed to prevent risk.
出处
《临床内科杂志》
CAS
2013年第8期561-563,共3页
Journal of Clinical Internal Medicine
基金
恩施州科技局2011年技术研究与开发项目计划[恩州科业（2011）13号]
