摘要
HPLC-NMR-MS联用技术在复杂混合物微量物质结构鉴定和代谢轮廓建立过程中内源性及外源性物质鉴定中的应用,表明其已成为天然产物活性物质药物化学、药理学及药代动力学研究中有用的分析工具。基于NMR技术的代谢组学方法可以精确刻画带有疾病特色的、包含各种疾病相关通路的综合代谢表型,可极大地丰富和补充传统的疾病评价指标,从而可用于天然产物活性物质毒理学研究、剂量优化、活性物质筛选及药效学评价等药理学研究领域。基于NMR技术的联用技术与代谢组学方法结合,将加快天然产物活性物质发现的速度,并提高其药理学评价的效率。
The application of HPLC-NMR-MS hyphenated technique in the structural identification of trace substances from complex mixtures and the identification of endogenous and exogenous substances in the establishment process of metabolic profiling have become effective analytical tools in pharmaceutical chemistry, pharmacological and pharmacokinetic studies of active substances from natural products. Metabolomics method based on NMR technology can accurately portray metabolic phenotypes with the characteristics of diseases and a variety of disease-related pathways, and it can greatly enrich and supplement the traditional disease evaluation methods. So it can be used for pharmacological studies of active substances from natural products, such as toxicological studies, the dose optimization, active substances screening and pharmacodynamic evaluation. Hyphenated technique associated with metabolomics method based on NMR technology will accelerate the speed of the discovery of active substances from natural products, and improve the efficiency of their pharmacological evaluation.
出处
《药学学报》
CAS
CSCD
北大核心
2013年第9期1383-1389,共7页
Acta Pharmaceutica Sinica
