摘要
为应对clade7.2 H5N1亚型禽流感病毒(AIV)持续发生的抗原性改变及其引发的潜在AIV的地方性流行,需要根据流行监测的抗原分析结果挑选合适的供体株制备疫苗候选株。本研究采用反向遗传操作系统,以A/Puerto Rico/8/34(PR8)的内部基因为骨架,以clade7.2 H5N1 AIV A/Chicken/Liao Ning/S4092/2011(CK/LN/S4092)基因组为模板经RT-PCR扩增其HA及NA基因,并对HA基因进行分子修饰,去除与H5亚型AIV致病力有关的HA蛋白裂解位点处的多个连续碱性氨基酸,使其获得低致病性AIV的分子特征(即将-PQIEGRRRKR-突变为-PQRETR-),从而构建出clade7.2 H5N1亚型AIV疫苗候选株rPR8-LN/S4092,为动物免疫试验奠定了基础。
To control of the continuing accumulation of antigenic changes within Clade 7.2 highly pathogenic avian influenza viruses （HPAIVs）, and the potential pandemic threat to poultry or even humans, it is necessary to update our vaccine stock according to the antigenic analysis based on surveillance. In this study, a reassortant virus, named rPR8-LN/S4092, was generated using reverse genetics. The virus contained modified HA and NA genes of an epidemic strain A/Chicken/Liao Ning/S4092/2011 in the background of internal genes derived form A/Puerto Rico/8/34 （PR8）. The reassortant virus was attenuated by removal of the multi-basic amino acid motif in the HA gene by mutation and deletion （from PQIEGRRRKR to PQRETR）, which could be served as a candidate vaccine virus.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2013年第9期764-766,共3页
Chinese Journal of Preventive Veterinary Medicine
