摘要
目的探讨永生化人支气管上皮细胞(human bronchial epithelial cells,BEAS-2B)端粒保卫蛋白1(protection of telomere 1,POT1)的表达下调与煤焦沥青(coal tar pitch,CTP)致端粒损伤的作用。方法 BEAS-2B细胞以CTP组,二甲基亚砜(dimethylsulfoxide,DMSO)溶剂对照组和空白对照组分别进行培养。培养至1代、10代、20代和30代时分别对细胞进行POT1基因RNA干扰,然后进行端粒长度、POT1和人端粒酶逆转录酶(human telomer-ase reverse transcriptase,hTERT)的mRNA及蛋白表达情况的检测。结果与对照组相比,抑制POT1基因的表达可导致hTERT的mRNA表达水平下调(F=25.008,P<0.001),端粒DNA相对长度缩短(F=14.617,P=0.001),随着培养代数的增加,端粒DNA相对长度缩短(F=15.988,P<0.001)。结论抑制POT1基因表达可间接促进CTP对BASE-2B细胞的端粒损伤作用。
Objective To explore the effect of down regulation of protection of telomere 1 （ POT1 ） and Coal Tar Pitch on the telomere damage of human bronchial epithelial cells（ BEAS-2B ） caused by CTP. Methods BEAS-2B cells were cultured respectively in CTP group, dimethylsulfoxide （DMSO） solvent control group and blank control group, and then, they were RNA interferenced in POT1 gene in the 1st, the 10th, the 20th and the 30th generations, the telomere length, the mRNAs expression of POT1 and human telomerase reverse transcriptase （hTERT） , and proteins expression of POT1 and hTERT were detected. Results Compared with control group, suppression of POT1 gene could reduce the mR- NA expression of hTERT（ F = 25. 008 ,P 〈 0. 001 ）, and the relative length of telomere DNA was shorterned（ F = 14. 617, P =0. 001 ）. With the increase of cell culture generation, the relative length of telomere DNA was shortern （F = 15. 988, P 〈 0. 001 ）. Conclusions Inhibiting the expression of POT1 gene can further the telomeredamage of BASE-2B cells by CTP.
出处
《中华疾病控制杂志》
CAS
北大核心
2013年第8期687-690,共4页
Chinese Journal of Disease Control & Prevention
基金
国家自然科学基金(81001239)
河南省教育厅自然科学研究计划(12A330003)
关键词
煤焦沥青
端粒
上皮细胞
RNA干扰
Coal tar pitch
Telomere
Epithelial cell
RNA interference
