摘要
目的 探讨平均红细胞体积 (MCV)测定法 (MCV法 )及红细胞脆性一管定量法 (一管法 )在筛查地中海贫血中的准确性及其应用价值。方法 用MCV法和一管法检查 989对夫妇 ,并随机抽取其中 79对进行聚合酶链反应 (PCR)法检测 ,同时检测孕妇血清铁蛋白以排除缺铁性贫血。结果 MCV法的轻型地中海贫血检出率为 9.0 %、敏感度为 98.9% ,一管法的轻型地中海贫血检出率为8.1%、敏感度为 88.6 % ,两者比较 ,差异有显著性 (P均 <0 .0 5 ) ;MCV法与一管法诊断轻型地中海贫血的特异度比较 ,差异无显著性 (P >0 .0 5 )。结论 MCV法与一管法均能有效地检出轻型地中海贫血患者 ,且MCV法对轻型地中海贫血具有较好的筛查。
Objective To evaluate and compare the detection efficiency of red cell mean corpuscular volume(MCV) test against simple tube red cell osmotic fragility quantitative test(STROFQT) in diagnosis of thalassemia. Methods 1978 subjects (989 couples) were detected by both MCV test and STROFQT, 158 samples from subjects were tested by α,β thalassemia DNA polymerase chain reaction(PCR) and serum ferritin test. Results The positive rate (9.0%), sensitivity(98.9%) of MCV test were higher than those of STROFQT (8.1%, 88.6%) ( P <0.05). The specificity of MCV test showed no statistic significance compared with that of STROFQT ( P >0.05). Conclusion Both MCV test and STROFQT served as helpful approaches in detection of thalassemia, MCV test shows better diagnostic efficiency for screening than STROFQT.
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2000年第10期610-612,共3页
Chinese Journal of Obstetrics and Gynecology
关键词
地中海贫血
产前诊断
红细胞指数
Thalassemia
Prenatal diagnosis
Mean corpuscular volumes
