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环丙沙星联合乳铁蛋白多肽嵌合体对铜绿假单胞菌毒力因子的影响 被引量：4

Effect of Ciprofloxacin Combined with Lactoferrin-derived Peptides Chimera on Virulence Factors of Pseudomonas aeruginosa
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摘要 目的研究环丙沙星(CIP)、乳铁蛋白多肽嵌合体(LFchimera)单用及二者联合对铜绿假单胞菌毒力因子生成的影响。方法以铜绿假单胞菌野生菌株PAO1为实验菌株,分为对照组(无任何干预的铜绿假单胞菌野生菌株PAO1),CIP(0.04μg/mL)组,LFchimera(0.25μmol/L)组,LFchimera(1μmol/L)组,CIP(0.04μg/mL)+LFchimera(1μmol/L)组,分别测定毒力因子绿脓菌素的浓度(氯仿萃取法)、弹性蛋白酶(弹性蛋白-刚果红法)、胞外蛋白水解酶(偶氮酪蛋白法)。结果和对照组比较,CIP组,LFchimera(0.25μmol/L)组,LFchimera(1μmol/L)组,CIP(0.04μg/mL)联合LFchimera(1μmol/L)组的绿脓菌素浓度下降,弹性蛋白酶和蛋白水解酶活性降低,差异均有统计学意义(均P<0.05);CIP组和LFchimera(0.25μmol/L)组比较,绿脓菌素浓度、弹性蛋白酶和蛋白水解酶活性的差异均无统计学意义(均P>0.05);LFchimera(1μmol/L)组绿脓菌素浓度、弹性蛋白酶和蛋白水解酶活性较CIP组和LFchimera(0.25μmol/L)组降低,差异均有统计学意义(均P<0.05);CIP(0.04μg/mL)联合LFchimera(1μmol/L)组和各单独用药组比较,绿脓菌素浓度下降,弹性蛋白酶和蛋白水解酶活性降低,差异均有统计学意义(均P<0.05)。结论亚抑菌浓度的CIP和LFchimera都对铜绿假单胞菌毒力因子(绿脓菌素、弹性蛋白酶和蛋白水解酶)的生成有抑制作用,随着LFchime-ra浓度的提高这种抑制作用加强,而CIP联合LFchimera能进一步增强这种抑制作用,说明两者在抗铜绿假单胞菌毒力因子方面有协同作用。 Objective To investigate the effect of ciprofloxacin（CIP）in combination with lactoferrin-derived peptides chimera（LFchimera）on virulence factors of Pseudomonas aeruginosa（P.aeruginosa）.Methods The wild type strain of P.aeruginosa-PAO1was used as experimental strain.Five groups were established,including control group（the wild type strain of P.aeruginosa-PAO1without any intervention）,CIP group（0.04μg/mL）group,LFchimera（0.25and 1μmol/L） groups,LFchimera（1μmol/L）plus CIP（0.04μg/mL）group.The virulence factors,including pyocyanin,elastase and extracellular proteolytic enzyme,were determined by chloroform extraction method,Elastin-Congo Red method and Azocasein method,respectively.Results The pyocyanin concentration,elastase activity and proteolytic enzyme activity were significantly reduced in the CIP group,0.25μmol/L LFchimera group,1μmol/L LFchimera group and CIP plus LFchimera group compared with those in the control group（P〈0.05）.There was no significant difference in the virulence factors between the CIP group and the 0.25μmol/L LFchimera group（P〈0.05）.Moreover,the level of the virulence factors was substantially reduced in the CIP plus LFchimera group when compared with 1μmol/L LFchimera group and CIP group（P〉0.05）.Conclusion Both CIP and LFchimera at sub-MICs can inhibit the production of virulence factors（such as pyocyanine,elastase and proteolytic enzyme）in Pseudomonas aeruginosa.They have synergistic effect on the resistance of virulence factors in Pseudomonas aeruginosa.
作者 左鹏 王正云 徐国鹏 胡琼洁 邵冰 熊维宁 熊盛道
机构地区 华中科技大学同济医学院附属同济医院呼吸与危重症医学科 南京医科大学附属苏州医院呼吸内科 华中科技大学同济医学院附属同济医院放射科 华中科技大学同济医学院护理学系
出处 《华中科技大学学报（医学版）》 CAS CSCD 北大核心 2013年第4期446-450,共5页 Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金 国家自然科学基金资助项目(No.30770946!) 中国呼吸道感染优化治疗协作组(CROTC)科研专项基金项目(No.2009042) 国家"十二五"支撑课题项目(No.2012BAI05B01)
关键词 铜绿假单胞菌 环丙沙星 乳铁蛋白 多肽 毒力因子 Pseudomonas aeruginosa ciprofloxacin lactoferrin peptides virulence factor
分类号 R453.2 [医药卫生—治疗学]
  • 相关文献

参考文献19

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共引文献23

同被引文献39

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华中科技大学学报（医学版）
2013年 第4期

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