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TWEAK对THP-1细胞中LOX-1表达的影响 被引量：2

Effect of TWEAK on LOX-1 Expression in THP-1Cells
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摘要 目的探讨肿瘤坏死因子样凋亡弱诱导剂(TWEAK)对人单核细胞(THP-1)中血凝素样氧化低密度脂蛋白受体-1(LOX-1)表达的影响。方法通过半定量RT-PCR检测不同浓度的TWEAK对THP-1细胞中LOX-1mRNA的表达,通过Western blot检测不同浓度的TWEAK对THP-1细胞中LOX-1蛋白表达的影响。结果发现TWEAK能够上调THP-1细胞中LOX-1mRNA及蛋白的表达,并且具有浓度和时间依赖性。结论 TWEAK可通过诱导单核-巨噬细胞表达LOX-1而具有致动脉粥样硬化的作用。 Objective To investigate the effect of TWEAK on LOX-1expression in THP-1cells.Methods The mRNA and protein expression of LOX-1was determined by semi-quantitative RT-PCR and Western blot,respectively,in THP-1cells treated with different concentrations of TWEAK.Results TWEAK could upregulate the expression of LOX-1at mRNA and protein levels in a dose-and time-dependent manner.Conclusion TWEAK is implicated in atherosclerosis through inducing LOX-1expression in monocytes/macrophages.
作者 吴勇波 陈志强 罗鹏程 曾和松
机构地区 湖北省黄石市中心医院(湖北理工学院附属医院)心血管内科 华中科技大学同济医学院附属同济医院心血管内科 湖北省黄石市中心医院(湖北理工学院附属医院)肾脏疾病发生与干预湖北省重点实验室
出处 《华中科技大学学报（医学版）》 CAS CSCD 北大核心 2013年第4期424-427,共4页 Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
关键词 动脉粥样硬化 肿瘤坏死因子样凋亡弱诱导剂 血凝素样氧化低密度脂蛋白受体-1 atherosclerosis TWEAK LOX-1
分类号 R543.5 [医药卫生—心血管疾病]
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参考文献16

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同被引文献36

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华中科技大学学报（医学版）
2013年 第4期

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