摘要
目的探讨缺血诱导因子-1α(HIF-1α)与血管内皮生长因子(VEGF)蛋白在原发性乳腺癌组织中的表达及与放疗疗效的关系。方法采用SABC免疫组化方法,检测乳腺癌组织(88例)和正常乳腺组织(20例)中HIF-1α与VEGF蛋白的表达。并分析HIF-1α或VEGF蛋白表达与原发性乳腺癌组织临床病理学特征和预后的关系。结果 HIF-1α与VEGF蛋白在原发性乳腺癌组织中的阳性表达率分别为69.32%(61/88)、78.40%(69/88),两者存在显著正相关性(r=0.68,P=0.006)。二者均与癌组织的临床病理学特征有关;27例HIF-1α阴性表达患者的生存时间明显长于61例阳性表达患者(P=0.008);19例阴性VEGF患者的生存时间明显长于69例阳性VEGF患者(P=0.006)。HIF-1α阳性表达患者中,VEGF表达强度及放疗后局部未控率均高于HIF-1α阴性组(P=0.01);其中16例VEGF阴性表达患者的生存时间明显长于42例VEGF阳性表达患者的生存时间(P=0.01)。结论 HIF-1α与VEGF蛋白表达可能与原发性乳腺癌的发生、浸润、转移有关,二者高表达与乳腺癌放射抗拒性有密切关系。
Objective To investigate the expression of HIF-1a and VEGF protein in primary breast carcinoma, and to evaluate its clinical significance in clinicopathological status and prognosis of patients with primary breast carcinoma. Methods SABC immunohistochemistry was used to assay the expression of HIF-1aor VEGF protein in the primary breast carcinoma patients （88 cases） or natural breast tissues （20 cases）, respectively. Results The positive expression rates of HIF-laand VEGF protein in breast carcinoma tissues were 69.32% （61/88） and 78.40% （69/88）, respectively. Clinicopathological parameters of breast carcinoma were associated with HIF-1 amnd VEGF protein expression. The positive rate of HIF-1 a was positively correlated with the expression of VEGF protein （r = 0. 68, P = 0. 006 ）. The positive expres- sion of HIF-1a x was closely related to clinical stage, prognosis and short - term effects of radiotherapy （P 〈 0.05 ）. Con- clusion HIF-1 ctand VEGF expression in cancerous tissues may play an important role in invasion and metastasis of prima- ry breast carcinoma. The expression of HIF-1 aand VEGF may serve as important parameters for evaluating the response to radiotherapy and establishing a prognosis for patients with primary breast cancer.
出处
《中南医学科学杂志》
CAS
2013年第4期368-371,共4页
Medical Science Journal of Central South China
