摘要
利用分子标记辅助选择和田间鉴定选择相结合的方法,将三黄占2号的抗稻瘟病主基因Pi-GD-1(t)、Pi-GD-2(t)和主效QTL GLP8-6(t)(分别简称G1、G2和G8)及抗白叶枯病基因Xa23导入到明恢86、蜀恢527和浙恢7954等3个骨干中籼恢复系,通过复交进行基因聚合,获得5个带有抗稻瘟病兼抗白叶枯病的双基因或多基因聚合系明恢86-G1-G2-Xa23、蜀恢527-G2-Xa23、明浙-G2-G8-Xa23-1、明浙-G2-G8-Xa23-2和明浙-G1-G2-G8-Xa23。以上5个抗病基因聚合改良系对稻瘟病的抗谱与抗源品种相仿或更宽,改良系和与不育系II-32A配制的测交种对白叶枯病菌的抗谱与供体亲本IRBB23一致,测交种在不接种白叶枯病菌条件下的产量和结实率与原来的恢复系及相应杂交种相仿,但在接种条件下带有Xa23基因的恢复系及测交种的结实率、千粒重和产量明显优于原来的恢复系及相应杂交种。研究表明,抗稻瘟病基因和抗白叶枯基因Xa23在不同恢复系背景下的抗性表达完全,对恢复系稻瘟病以及白叶枯病改良的效果明显。
The novel blast resistance genes, Pi-GD-I（t）, Pi-GD-2（t）, and GLP8-6（t） from rice variety Sanhuangzhan 2 （here ab- breviated them as G 1, G2, and G8, respectively） and bacterial blight （BB） resistance gene, Xa23, were introgressed into three elite restorer lines （Minghui 86, Shuhui 527, and Zhehui 7954） by marker-assisted pyramiding breeding approaches in combination with artificial inoculation and stringent phenotypic selections. The five derived blast and bacterial blight resistance restorer lines, Minghui 86-G I-G2-Xa23, Shuhui 527-G2-Xa23, Mingzhe-G2-G8-Xa23-1, Mingzhe-G2-G8-Xa23-2, and Mingzhe-G1-G2-G8- Xa23, demonstrated similar or wider blast resistance spectrum as compared with the donor parent, Sanhuangzhan 2. The five de- rived BB resistant restorer lines and their derived hybrid combinations with II-32A demonstrated similar BB resistance spectrum to the donor parent, IRBB23. The newly developed BB resistant restorers and their derived hybrids were identical to their respec- tive original versions for agronomic traits especially under disease free condition. However, under severe disease condition, the five BB resistant restorer lines exhibited significantly higher grain weight, spikelet fertility, and grain yield as compared with the respective original restorer lines, thus further resulting in significantly higher grain yields in BB resistant hybrids than in their respective original hybrids.
出处
《作物学报》
CAS
CSCD
北大核心
2013年第9期1582-1593,共12页
Acta Agronomica Sinica
基金
引进国际先进农业科学技术计划(948计划)(2011-G2B)
国家高技术研究发展计划(863计划)(2012AA101101)
浙江省公益技术研究项目(2012C22033)资助
关键词
水稻恢复系
稻瘟病
白叶枯病
抗性改良
标记辅助选择
Rice restorer line
Blast
Bacterial leaf blight
Resistance improvement
Marker assisted selection （MAS）
