摘要
目的 探讨造血干细胞冷冻保存时间与细胞活性之间的关系。方法 造血干细胞中加入细胞冷冻保护剂 ,通过程控冷冻 ,放入液氮保存。根据保存的时间不同 ,复苏后测定细胞活力的变化。结果 细胞液氮保存复苏后 ,绝大部分的细胞活力得以保存。结论 造血干细胞贮存在液氮中 ,深低温阻断了酶的活性和细胞代谢 ,贮存时间长短与细胞活性无关。
Objective To explore the correlation between the duration of hemotopoietic stem cell freezing preservation and cell viability.Method Hemotopoietic stem cell were mixed with protective agent of freezing and preseved in liquid nitrogen with programmed freezing.The cell viability was determined after different time of preservation.Results After freezing preservation,the viability of the most of the cells was preserved.Conclusion During freezing preservation of hemotopoietis stem cells in liquid nitrogen,deep hypotemperature blocks the activity of enzyme and cell metabolism and there is no relationship between preservation time and cell viability.
出处
《苏州医学院学报》
2000年第9期809-810,共2页
Acta Academiae Medicinae Suzhou
关键词
恶性血液病
造血干细胞
冷冻保存
细胞活力
hemotologic malignancies
hemotopoietic stem cell
freezing preservation
cell viability
