摘要
利用现代分子生物学技术扩增获得草菇组织分离物与草菇出发菌株的RAPD和ITS的DNA指纹图谱 ,以此估算两者的DNA相似系数 ,并构建了遗传相关聚类图。结果表明 ,两者存在着一定的遗传差异。
The amplified DNA fingerprints from RAPD and ITS of Volvariella volvacea tissue isolates and parental strains were obtained by modern molecular biological techniques. The DNA similarity coefficients of V. volvacea strain and tissue isolate were estimated and cluster dendrogram was constructed based on the DNA fingerprints. The results showed that genetic variation existed between these two strains.
出处
《食用菌学报》
2000年第2期1-4,共4页
Acta Edulis Fungi
基金
上海市农委科技兴农重点攻关项目
关键词
草菇
组织分离
遗传变异
遗传稳定性
Volvariella volvacea
Tissue isolate
Genetic variatio(