摘要
在（１３±１）℃／（７±１） ℃的低温条件下研究了不同处理时间对番茄苗期和坐果期 ＰＯＤ活性及其同工酶的影响。结果表明：随处理时间延长，番茄叶片中的 ＰＯＤ活性增高，苗期在处理６ｄ后增加幅度较大，坐果期 ＰＯＤ活性低于苗期。低温处理苗期耐低温品系６ｄ时出现一条Ｒ_ｆ＝０．６２５的新酶带，而不耐低温品系处理１０ｄ后才出现；低温处理坐果期番茄叶片无新酶带的出现，只表现为活性的增强。苗期 Ｒ_ｆ＝ ０．６２５， ０．６３７５；坐果期 Ｒ_ｆ＝０．６， ０．６２５的同工酶谱带与番茄的耐低温性有关。番茄苗期与坐果期对低温的敏感程度近似相同，但坐果期比苗期耐低温能力弱。
The effect of different low temperature(13± l) ℃ /(7± l)℃ treatment time on Peroxidase (POD) activities and its isoenzyme during seedling stage and fruit-set period was studied. The results showed that POD activities were increased by low temperature in tomato leaves. During seedling Stage, POD activities had a rapid increase after 6d. treatment; a new isoenzymic band appeared (R, =0.625) after ed. treatment in low temperature tolerant line, but the bend appeared after 10d. treatment in low temperature sensitive line. POD activities in fruit-set period were lower than that in seedling stage. There was no new isoenzymic band during fruit--set period, but 2 isoenzymic bands intensities were increased by low temperature. There was a relationship between boenzymic bands (R_f =0.625p0.6375 in Sealling stage; R_f=0.6p0.625 in fruit--set period) and low temperature tolerance in tomato. The sensitivity to temperature was same for seedling stage and fruit--set period, approximately. Bat the low temperature tolerance of fruit--set period was lower than that in seedling stage.
出处
《沈阳农业大学学报》
CAS
CSCD
2000年第1期47-49,共3页
Journal of Shenyang Agricultural University
关键词
番茄
苗期
坐果期
POD
同工酶
低温冷害
tomato
seedling stage and fruit-set period
POD activity
isoenzyme
