摘要
利用真菌DNA-ITS区序列中的通用引物ITS1和ITS4,以黄瓜棒孢叶斑病菌的基因组DNA为模板,进行PCR,扩增出1条600 bp大小的条带。进行序列分析后发现其为黄瓜棒孢叶斑病菌的基因序列。根据黄瓜棒孢叶斑病菌(Corynespora cassiicola)与其他黄瓜病原真菌ITS序列间的差异,设计了1对特异性引物CCC1/CCC2,用于黄瓜棒孢叶斑病的种子带菌分子检测,从而对来自北京郊区黄瓜种子上的棒孢叶斑病的带菌情况进行了检测,发现来自房山和通州的黄瓜种子均在约600 bp出现特异性条带,说明黄瓜种子中携带棒孢叶斑病菌。
A DNA band of sequence about 600bp was got from Corynespora cassiicola genome DNA by PCR assay with universal primer of ITS1/ITS4 in Fungi rDNA-ITS （internal transcribed spacer） and sequence assay in NCB1. Based on dif- ferences between ITS sequences in C. cassiicola and that in other cucumber fungal pathogens, a pair of species-specific primers CCC1/CCC2 was designed for its detection, which was used to detect bacteria of the disease in cucumber seed in Fangshan and Tongzhou districts in Beijing. The results showed that the cucumber seeds carried target spot pathogen be- cause of appearance of specific band about 600 bp by PCR.
出处
《中国植保导刊》
北大核心
2013年第8期13-17,共5页
China Plant Protection
基金
北京农业职业学院院级课题:北京市农业局农业科技新星支持项目(DXM-2012-036203-000012)
