摘要
以葡萄糖为碳源利用A.succinogenes NJ113生产丁二酸时,副产物乙酸较高,以A.succinogenes NJ113为出发菌,采用自主设计的连续培养装置,筛选利用甘油能力较强的突变株,最终筛选到一株能够利用浓度达到8 g/L的突变株ZH99。此突变株在以27 g/L的葡萄糖和3 g/L的甘油作为碳源,在血清瓶中厌氧发酵,丁二酸产量达到20.81g/L,比原始菌株的丁二酸的量20.42 g/L稍有提高,同时,乙酸的浓度仅为5.02 g/L,比对照组(仅以葡萄糖为碳源)降低了38.25%,效果显著。
Fermentation of glucose on succinic acid in A. succinogenes NJ113, the concentration of acetic acid as byproduct was high. Therefore, A. succinogenes NJ113 was selected as the parent strain, which was mutagenized by continuous- culture technique in which all environmental factors beside the stress （glycerol concentration） were maintained constant, aiming at isolating a glycerol - strong mutant of A. succinogenes for analyzing the effects of co- fermentation of glucose and glycerol on acetic acid formation. In this technique, a mutant strain ZH99, could grow in the concentration glycerol of 8 g/L, was obtained after continuous - culture of 526 h. The results showed that the mu- tant ZH99 could produce succinic acid 20.81 g/L, while the acetic acid production was only 5.02 g/L, which de- creased by 38.25% in the condition of 27 g/L glucose and 27 g/L glycerol , compared to the parent strain.
出处
《化工时刊》
CAS
2013年第7期1-4,共4页
Chemical Industry Times
