摘要
为了建立蛇莓RAPD反应的最佳反应体系,我们对每个反应因子进行优化研究。在本实验室常用的RAPD反应体系的基础上,根据RAPD扩增效果确定蛇莓基因组DNA的最佳RAPD反应体系。经优化后,我们建立了适合蛇莓的最佳RAPD反应体系:25μL反应体系中含10×Buffer 2.5μL,2 mmol/L Mg2+,1.5 UTaqDNA聚合酶,50 ng模板,0.22 mmol/L dNTPs,0.35μmol/L随机引物S30。本研究结果为下一步野生蛇莓遗传多样评估奠定基础。
In order to establish the optimal RAPD reaction system in Duchesnea indica, every factor in the system was optimized. Based on the RAID reaction system commonly used in our lab, the optimal RAPD reaction system of genome DNA of D. indica was determined according to the RAPD amplification effects. The optimal PCR system was as follows： 2.5 μL 10×buffer, 2.0 mmol/L Mg^2＋, 1.5 U Taq polymerase, 50 ng DNA template, 0.2 mmol/L dNTPs, 0.35 μmol/L random primer S30 in 25μl reaction system. The results of this paper will lay the basis for assessing the genetic diversity of the wild D. indica.
出处
《井冈山大学学报（自然科学版）》
2013年第4期37-40,共4页
Journal of Jinggangshan University (Natural Science)
基金
井冈山大学科研项目(JZ0821)
关键词
蛇莓
RAPD
扩增程序
Duchesnea indica
RAPD
amplification procedure
