摘要
采用固相萃取(solid phase extraction,SPE)前处理结合高效液相色谱荧光检测(high performance liquid chromato graphy fluorescence detection,HPLC-FD),建立了检测吸烟人群尿中8种羟基多环芳烃(OHpolycyclic aromatic hydrocarbons,OH-PAHs,环数2～5)的SPE-HPLC-FD分析方法;在优化后的SPE和HPLC-FD条件下,内标法定量检测尿中8种OH-PAHs.结果表明,标准曲线方程的线性范围为0.2～20μg/L或0.5～20μg/L,决定系数R20.998,方法定量限(method quantification limit,MQL)为0.03～0.07μg/L,回收率R为(70%±4%)～(109%±30%),日间重现性为5%～28%.当地吸烟者尿样分析结果显示,本方法能够检测其尿中不同环数的OH-PAHs,可用于当地吸烟人群PAHs暴露健康风险评价.
To assess the exposure to polycyclic aromatic hydrocarbons （PAHs） by smoking, a method to detect 8 types of urinary hydroxylated metabolites of PAHs （OH-PAHs） （containing 2 to 5 rings） was developed by using solid phase extraction （SPE） coupled to high performance liquid chromatography fluorescence detection （HPLC-FD）. With optimized SPE and HPLC-FD conditions, 8 standard curve equations were established for individual OH-PAHs with linear ranges of 0.2-20μg/L or 0.5-20 μg/L （all determination coefficients R2 ≥ 0.998）. The method quantification limits （MDLs） were 0.03-0.07 μg/L, and recoveries R were （70% ± 4%）-（109%±30%）, while inter-day variations ranged from 5% to 28%. OH-PAHs in urine samples from local smokers were successfully detected, showing that the method is promising in assessing exposure to PAHs for local smokers.
出处
《上海大学学报（自然科学版）》
CAS
CSCD
北大核心
2013年第4期374-379,共6页
Journal of Shanghai University:Natural Science Edition
基金
国家自然科学青年基金资助项目(21107066)
上海高校青年教师培养资助计划项目
上海大学创新基金资助项目
关键词
羟基多环芳烃
生物标志物
尿样
吸烟人群
健康风险评价
hydroxylated metabolite of polycyclic aromatic carbons
biomarker
urine
smoker
health risk assessment
