摘要
赤胸梳爪步Dolichus halensis(Schaller)(鞘翅目:步甲科)是重要的捕食性天敌昆虫,在我国分布广泛。为揭示其种群遗传多样性和扩张机制,本研究以秦岭地区为中心,以线粒体Cox1-tRNALeu-Cox2基因片段为分子标记,对来自于24个采集点共191个个体进行了检测分析。在长度为1 601 bp的碱基中共检测到45个变异位点,定义了53个单倍型,单倍型多样性高(H d=0.796),而核苷酸多样性较低(P i=0.0033)。系统发育分析结果表明该地区该物种存在两大进化枝。分子变异分析(AMOVA)表明86.61%的变异来源于种群内。SAMOVA和PERMUT分析结果一致,表明秦岭地区分布的赤胸梳爪步甲种群不存在明显的谱系地理结构。中性检验和错配分布分析的结果一致,表明该物种在秦岭地区曾经发生过种群扩张。综上,认为赤胸疏爪步甲种群经历过冰期后的扩散。
halensis （Schaller） （Coleoptera： Carabidae） is an important predator insect that is widely distributed in China. To reveal the genetic diversity and demographic history of D. halensis populations from the Qinling Mountains, a 1 601 bp fragment from the mtDNA gene （Cox1-tRNALeu-Cox2） was sequenced for 191 individuals from 24 local populations. Forty-five polymorphic sites were found, and 53 haplotypes were identified. The high haplotype diversity （Hd=0.796） was accompanied by lower nucleotide diversity （Pi=0.0033）. Phylogenetic analysis （Bayesian inference） of the 53 haplotypes revealed two major clades. AMOVA analysis suggested that most of the variation occurred within populations （86.61%）. SAMOVA and PERMUT analyses showed no phylogeographic structure. The results of neutrality tests and mismatch distribution analyses showed a sudden population expansion. The above analyses confirmed the existence of a postglacial population expansion.
出处
《昆虫学报》
CAS
CSCD
北大核心
2013年第7期807-815,共9页
Acta Entomologica Sinica
基金
山西师范大学博士启动金(833237)
山西师范大学校基金(ZR1219,SD2008YBKT-011)
关键词
赤胸疏爪步甲
线粒体DNA
遗传多样性
种群扩张
秦岭
Dolichus halensis mitochondrial DNA genetic diversity population expansion Qinling Mountains
