摘要
用1.95×10-4 mol.L-1的KK-42处理日本沼虾1min,不同时刻抽取血淋巴、解剖肝胰腺.HPLC测定血淋巴中20E滴度,RT-PCR测定EcR和RXR mRNA水平.结果:KK-42处理后3h,6h,20E滴度分别比对照组增加了121.71%(P<0.05)、268.96%(P<0.01);EcR mRNA水平在处理后6h,12h,分别比对照组提高了233.56%(P<0.01)、110.73%(P<0.05);RXR mRNA水平在处理后3h、12h,分别比对照组提高了195.53%(P<0.01)、80.83%(P<0.05).表明KK-42可显著提高血淋巴中20E的滴度,并诱导肝胰腺中EcR和RXR的基因转录.
The spawns were soaked for 1 min in KK-42 solution at a concentration of 1.95 X 10-4 mol L-1 , and then hemolymph was extracted, and hepatopancreas were removed. Hemolymph 20 E titer was determined by HPLC. EcR and RXR mRNA level were assayed by RT PCR. The result showed that the titers of hemolymph 20 E significantly increased after KK- 42 treatment compared with the corresponding controls at 3 h（P〈0.05） and 6 h（P 〈0.01） ; the hepatopancreas F.cR mRNA level significantly increased compared with the corresponding controls at 6 h（P〈0.01） and 12 h（P〈0.05） after KK-42 treat- ment; the hepatopancreas RXR mRNA level significantly increased compared with the corresponding controls at 3 h（P〈0.01） and 12 h（P〈0.05）after KK-42 treatment. It indicates that KK-42 treatment enhanced ecdysone titer, and up-regulatecl the ex- pressions of EcR and RXR.
出处
《河南师范大学学报（自然科学版）》
CAS
北大核心
2013年第4期124-127,共4页
Journal of Henan Normal University(Natural Science Edition)
基金
河南省高校科技创新人才支持计划(2009HASTIT022)
