摘要
目的比较汽油汽车燃烧尾气和甲醇汽车燃烧尾气对肺巨噬细胞免疫功能的影响,为筛选清洁能源提供依据。方法利用肺巨噬细胞受体能与致敏的绵羊红细胞反应形成玫瑰花环并有一定的结合率,将灌洗出的兔肺巨噬细胞培养一定时间后再按0.500、0.250、0.125、0.062、0.031 L/ml浓度将汽油和甲醇燃烧尾气的提取物对巨噬细胞染毒,在37℃、5%CO2孵箱中培养3 h后加入致敏的绵羊红细胞,放入冰箱内反应2 h。取出后计数玫瑰花环形成率。并进行了抗体介导的细胞毒作用试验。结果汽油和甲醇燃烧尾气作用都使巨噬细胞花环形成率降低,未染毒组的结合率为85.7%,汽油和甲醇在最低试验浓度0.031 L/ml时花环结合率分别为47.0%和70.0%,汽油燃烧尾气结合率明显低于甲醇燃烧尾气;汽油尾气提取物对抗体介导的细胞毒作用有显著的抑制作用,汽油燃烧尾气使其降低的幅度明显大于甲醇燃烧尾气。结论甲醇燃烧尾气对巨噬细胞的免疫功能的影响明显小于汽油燃烧尾气,所以更适合做清洁能源。
[ Objective ] To compare the effects between gasoline automobile exhaust gas and methanol automobile exhaust gas on the immune function of pulmonary macrophage, provide the basis for the selection of clean energy. [ Methods ] Based on the reaction that pulmonary macrophage receptor can present the rosettes combined with sensitized sheep erythrocytes with the different formation rate, the rabbit lung macrophages cultured for a certain period of time after lavage were exposed to gasoline and methanol exhaust gas extract according to the following concentration of 0. 500 L/ml, 0. 250 L/ml, 0. 125 L/ml, 0. 062 L/ml and 0. 031 L/ml, and were stored at 37 ℃ in 5% CO2 incubator for 3 hours. The sensitized sheep erythrocytes were added to the culture, placed in the refrigerator for 2 hours. The formation rate of rosettes was counted, and the antibody-dependent cell-mediated cytotoxicity test was conducted. [ Results] Both gasoline and methanol exhaust gas can reduce the formation rate of macrophage rosettes, and the formation rate was 85.7% in non-exposure group. The formation rate of the gasoline group and methanol group in the minimum concentration of 0. 031 L/ml was 47.0% and 70.0% respectively, which the formation rate of gasoline exhaust gas was significantly lower than that of methanol exhaust gas. The gasoline exhaust gas extract had obvious inhibitory effect on the antibody-dependent cell-mediated cytotoxicity, and the decrease range of this test by the gasoline exhaust gas was significantly larger than that by methanol exhaust gas. [ Conclusion] The effect of methanol exhaust gas on the immune function of macrophages is significantly less than that of gasoline exhaust gas, so the methanol is more suitable for the clean energy.
出处
《职业与健康》
CAS
2013年第17期2162-2164,共3页
Occupation and Health
关键词
肺巨噬细胞
玫瑰花环
免疫活性
汽油
甲醇
Pulmonary macrophage
Rosettes
Immune activity
Gasoline
Methanol
