摘要
目的:建立HPLC同步测定天舒胶囊中天麻素、5-羟甲基糠醛、阿魏酸3种成分的含量。方法:Phenomenex LunaC18色谱柱(4.6 mm×250 mm,5μm),甲醇(A)-0.1%磷酸水溶液(B)梯度洗脱(0～10 min,5%～10%A;10～16 min,10%A;16～50 min,10%～60%A),流速1.0 mL·min-1,检测波长221 nm(天麻素)、283 nm(5-羟甲基糠醛)、322 nm(阿魏酸),柱温30℃。结果:天麻素、5-羟甲基糠醛、阿魏酸分别在5.61～299,3.31～212,4.63～247 mg·L-1呈良好的线性关系;天舒胶囊中3种成分的加样回收率分别为99.74%,102.5%,100.3%,RSD分别为0.84%,1.53%,0.49%。5批天舒胶囊市售样品中天麻素、5-羟甲基糠醛和阿魏酸的含量分别为2.70～3.65,2.07～2.67,1.95～2.10 mg·g-1。结论:测定天麻素、5-羟甲基糠醛和阿魏酸的含量能够有效地控制天舒胶囊的产品质量。
Objective：To develop a HPLC method for the simultaneous determination of gastrodin,5hydroxymethylfurfural and ferulic acid in Tianshu Capsule.Method：Phenomenex Luna C18column（4.6 mm × 250 mm,5 μm） was used.The mobile phase consisted of methanol（A） and water containing 0.1% phosphoric acid（B）,and the gradient program was as followed：0-10 min,5%-10% A;10-16 min,10% A;16-50 min,10%-60% A.The flow rate was 1.0 mL·min-1 and the column temperature was set at 30 ℃.The detection wavelength was at 221 nm（gastrodin）,283 nm（5-hydroxymethylfurfural） and 322 nm（ferulic acid）.Result：The linear ranges were 5.61-299 mg·L-1 for gastrodin,3.31-212 mg·L-1 for 5-hydroxymethylfurfural and 4.63247 mg·L-1 for ferulic acid,respectively.The average recoveries of three constituents were 99.74%,102.5% and 100.3% with the RSD of 0.84%,1.53% and 0.49%.The contents of three compounds,gastrodin,5hydroxymethylfurfural and ferulic acid in Tianshu Capsules were 2.70-3.65,2.07-2.67 mg·g-1 and 1.95-2.10 mg·g-1.Conclusion：Determination of gastrodin,5-hydroxymethylfurfural and ferulic acid could effectively control the product quality of Tianshu Capsules.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第17期81-84,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
中国博士后基金项目(2012M510731)
中药制药过程新技术国家重点实验室开放基金项目(SKL2010M0204)
重大新药创制项目(2009ZX09308-003)
