摘要
对黄檗落叶中新化感物质黄柏苷在黄檗及近缘种黄皮树种子萌发及生长过程中的化感作用进行研究,以期解读黄檗野生种群中幼苗稀少的成因。从黄檗落叶中提取分离黄柏苷,用不同浓度的黄柏苷溶液对后熟阶段及发芽阶段的黄檗及黄皮树种子进行处理,检测种子发芽率、发芽指数、幼苗株高及根的长度,通过各单项指标及综合性指标(种子活力指数)评价黄柏苷的化感效应。结果表明:黄柏苷溶液在0.30 g·L-1水平对黄檗种子的后熟、0.15 g·L-1水平对黄檗种子的萌发产生显著抑制作用;在0.15 g·L-1水平对黄皮树种子的后熟及萌发均产生显著抑制作用,且抑制强度随浓度的增高而增强。黄柏苷的存在可降低黄檗及黄皮树种子的发芽率,延长萌发时间,降低幼苗对环境的适应能力,黄柏苷可能是黄檗野生种群中幼苗稀少的成因之一。
Conducted research on new allelochemicals phellamurine extracted from deciduous of Phellodendron amurense, which worked in allelopathy effect to seed germination and growth process of P. amurense and P. chinense in order to interpret the cau- ses of rare seedlings of wild populations of P. amurense. Extracted and separated phellamurine from P. amurense deciduous, and trea-ted the seeds of P. amurense and P. chinense in after-ripening stage and germination stage with different concentrations of phellamurine solution, then detection of the seed germination rate, germination index, seedling height, root length and seed vigor index to evaluate the allelopathic effect of phellamurine. The results show that： phellamurine solution at 0. 30 g·L^-1 produce significant inhibition to seed after-ripening of P. amurense, and also the solution at 0. 15 g·L^-1 produce significant inhibition to seeds germination of P. amu-rense; the solution at 0. 15 g·L^-1 produce significant inhibition to seeds after-ripening and seeds germination of P. chinense, inhibi-tion intensity increased with the concentration and enhanced. For both species, the presence of phellamurine can lower the seed germi- nation rate, extend the germination time, reduce the ability of seedlings to adapt to the environment, thus the phellamurine may be one of the causes of rare seedlings in the wild population of P. amurense.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2013年第17期2768-2772,共5页
China Journal of Chinese Materia Medica
基金
国家"重大新药创制"科技重大专项(2012ZX09304006)
关键词
黄柏苷
化感作用
黄檗
黄皮树
种子萌发
phellamurine
allelopathy
Phellodendron amurense
Phellodendron chinense
seed germination
