摘要
[目的 ]验证参与卡氏肺孢子虫致病过程中 ,宿主炎性反应及其对肺功能的影响。 [方法 ]:将小鼠CD4+ T细胞耗竭或将小鼠CD4+ 和CD8+ T细胞都耗竭后 ,经气管接种卡氏肺孢子虫。观察在CD8+ T细胞缺如或存在的情况下 ,小鼠呼吸机能的改变和肺炎的程度。 [结果 ]耗竭CD4+ 和CD8+ T细胞后 ,小鼠虽发生PCP ,但呼吸频率无明显加快 ,肺组织损伤程度也较轻。相反 ,仅耗竭CD4+ T细胞保留CD8+ T细胞的小鼠的呼吸频率明显加快 ,肺内炎性细胞反应和肺组织损伤程度均较重。支气管肺泡灌洗液 (BALF)中的CD8+ T细胞和嗜中性白细胞的数量明显增多。 [结论 ]PCP的致病过程中 ,宿主的炎性细胞反应对肺功能有直接损伤作用 ,其中CD8+ T细胞似起主要作用。
Objective] To define the cell populations contributing to inflammation-associated respiratory impairment in Pneumocystis carinii pneumonia (PCP). [Methods] The host inflammation response was observed in CD4 +T cell-depleted mice and in CD4 + and CD8 + T cell-depleted mice infected with P carinii via intratracheal inoculation. [Results] Mice depleted of both CD4 + and CD8 + T cells developed infection with neither increased respiratory rate nor lung injury. In contrast, mice depleted of CD4 + T cells alone exhibited severe pulmonary inflammation, and increased respiratory rates. Respiratory compromise was associated with the presence of activated CD8 + cells and neutrophils in the BALF. [Conclusion] The host′s inflammatory cell response to P. carinii directly impairs pulmonary function and contributes to the pathogenesis of PCP, CD8 + T cells appear to play a major role.
出处
《中国寄生虫学与寄生虫病杂志》
CSCD
北大核心
2000年第4期207-212,共6页
Chinese Journal of Parasitology and Parasitic Diseases
