摘要
为探讨Ca2+、Mg2+、盐度对凡纳滨对虾体内代谢酶的相对独立作用和相互影响,进而为提高凡纳滨对虾生长力和免疫力提供理论依据,本实验采取L49(78)安排7水平Ca2+、Mg2+、盐度,L8(27)安排2水平Ca2+、Mg2+、盐度,开展60 d凡纳滨对虾(Litopenaeus vannamei)养殖试验,通过比较对虾体内消化酶、ATP酶及免疫类酶的活性以分析Ca2+、Mg2+、盐度对凡纳滨对虾生长力和免疫力的影响。结果表明:水体中Ca2+、Mg2+、盐度对消化酶具有显著影响(P<0.05),其中与对虾消化吸收联系最紧密的蛋白酶中,盐度对胃蛋白酶影响显著,盐度为10时酶活最高,Ca2+、盐度对类胰蛋白酶影响显著,Ca2+为200 mg/L,盐度为20时,酶活最高;Ca2+、Mg2+、盐度对ATP酶具有显著影响(P<0.05),其中对Na+-K+-ATP酶都有显著影响,Ca2+为300 mg/L,Mg2+为500 mg/L,盐度为30时酶活最高,Ca2+、Mg2+对Mg2+-ATP酶具有显著影响,Ca2+为200 mg/L,Mg2+为500 mg/L时酶活最高,Ca2+对Ca2+-ATP酶具有显著影响,Ca2+为200、300 mg/L时酶活最高;Ca2+、Mg2+、盐度对凡纳滨对虾体内免疫酶具有显著影响(P<0.05),Ca2+、Mg2+、盐度对ACP都有显著影响,Ca2+为100 mg/L,Mg2+为150 mg/L,盐度为30时酶活最高,Mg2+对AKP具有显著影响,在150 mg/L时酶活最高,Ca2+、盐度对SOD酶活具有显著影响,Ca2+为100 mg/L,盐度为35时酶活最高;Ca2+、Mg2+、盐度间的交互作用对体内代谢酶也有一定影响。
The L49（7^8） orthogonal experimental design was used to investigate the three factors Ca^2＋, Mg^2＋ and salinity in water, and seven levels of each factor were based. The L49（78） orthogonal experimental design was used to investigate the three factors Ca^2＋, Mg^2＋ and salinity in water, and two levels of each factor were based. In order to o analyze the effect of Ca^2＋, Mg^2＋ and salinity in water on the growth performance and immunity ofL. vannamei, the digestive enzymes, ATP enzyme and immune enzymes were compared during the 60 d culture cycle. Results showed that Ca^2＋, and salinity had significant effect on digestive enzymes（P〈0.05）, the salinity naa slgnlncant effect on pepsin, and the Ca^2＋ and salinity had significant effect on tgryptase. Ca^2＋, Mg^2＋ and salinity had significant effect on ATP enzymes（P〈0.05）, and Ca^2＋, Mg^2＋ and salinity had significant effect on Na^＋-K^＋-ATPase, with maximum activity at Ca^2＋ 300 mg/L, Mg^2＋ 500 mg/L and salinity 30. Ca^2＋ and Mg^2＋ had significant effect on Mg^2＋-ATPase and Ca^2＋ had significant effect on Ca^2＋-ATPase. Ca^2＋, Mg^2＋ and salinity had significant effect on immune en- zymes（P〈0.05）, and Ca^2＋, Mg^2＋ and salinity had significant effect on ACP, with maximum activity at Ca^2＋ 100 mg/L, Mg^2＋ 150 mg/L and salinity 30. Mg^2＋ had significant effect on AKP with maximum activity at 150 mg/L. Ca^2＋ and salinity had significant effect on SOD, with maximum activity at Ca^2＋ 100 rag/L, salinity 35. The interaction of Ca^2＋, Mg^2＋, and salinity also had some impact on metabolic enzymes of L. vannamei.
出处
《海洋科学》
CAS
CSCD
北大核心
2013年第7期63-71,共9页
Marine Sciences
基金
上海市科技兴农重点攻关项目(沪农科攻字(2010)第1-6号)
上海市科技兴农科技推广项目(沪农科推字(2008)第5-1号)
上海市教育委员会重点学科建设项目(J50701)
