摘要
目的:从蒙药那仁满都拉中分离多糖,进行定性分析并研究其抗氧化活性。方法:采用水提醇沉法提取多糖,Sevag法脱蛋白、H2O2脱色、透析、再通过DEAE-Cellulose、Sephdex G-100柱色谱进一步纯化,通过比色法测定多糖对氧自由基的清除及对脂质过氧化物(LPO)的抑制作用。结果:蒙成药那仁满都拉粗多糖的含量为61.9%,纯化后多糖的含量为91.7%。多糖对.OH的清除能力弱于苯甲酸,但强于维生素C和甘露醇;对.O2-的清除能力弱于苯甲酸和维生素C,强于甘露醇。当多糖浓度达到一定时可有效降低LPO的终产物——丙二醛(MDA)的含量。结论:该蒙药多糖对.OH和.O2-具有较强的清除作用,其活性大小与浓度呈正效应;对脂质过氧化物具有一定的抑制作用。
Objective： To study the isolation and qualitative analysis and antioxidant activity of polysaccharides from Mongolian medicine Narenmandula.Methods： Polysaccharides were extracted by water extraction and alcohol precipitation,then processed by deproteinization with Sevag method,decolorization with H2O2,and dialysis,then were further purified by through DEAE-Cellulose and SephdexG-100 column chromatography.The scavenging effect on oxygen free radicals and inhibition of lipid peroxidation（LPO） of polysaccharides were analyzed by the colorimetric method.Results： The content of crude polysaccharides was 61.9%,and the content of purified polysaccharides was 91.7% in Mongolian medicine Narenmandula,respectively.The scavenging effect of polysaccharides on.OH was weaker than that of benzoic acid,but stronger than that of Vc and mannitol,and polysaccharides had a weaker scavenging effect on.O2-than benzoic acid and Vc,but stronger than mannitol.When the polysaccharide concentration reached a certain level,it could effectively reduce the content of malondialdehyde（MDA）,the final product of LPO.Conclusion： The polysaccharide of Mongolian medicine has a strong scavenging effect on.OH and.O2-,whose activity is positively correlated with concentration;and it can inhibit lipid peroxidation at certain degree.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2013年第8期1285-1288,共4页
Chinese Journal of Pharmaceutical Analysis
基金
内蒙古自然科学基金项目(2011MS0212)
国家自然科学基金资助项目(81260682)
关键词
蒙药
那仁满都拉
多糖
纯化
抗氧化
活性研究
Mongolian medicine
Narenmandula
polysaccharide
purification
antioxidant activity
activity studies
