摘要
目的:探讨小分子药物维生素C(VC)对CD4+效应记忆性T细胞(TEM)体外扩增的影响从而改善过继免疫治疗的效果。方法:体外分离正常人的外周血CD4+T淋巴细胞,将细胞分为2组(实验组和对照组)进行细胞培养。实验组加入VC后,通过细胞计数仪和流式细胞仪对2组细胞中TEM的扩增情况进行检测。结果:(1)在CD4+T细胞体外扩增实验中,VC对CD4+T细胞的总数无显著影响。(2)VC使TEM在CD4+T细胞中的比例显著升高,100 mg/L为扩增的最佳浓度。(3)在CD4+T细胞扩增的第10天检测其中TEM的细胞数量,对照组TEM的数量为(1.22±0.15)×106,实验组TEM的数量为(3.56±0.35)×106,两者的差异有统计学意义(P<0.01)。结论:VC可有效地促进CD4+TEM的体外扩增,为过继免疫治疗提供简单、安全及有效的体外扩增方法。
AIM： To investigate the effect of vitamin C （VC）, a small-molecule compound, on the expansion of CD4＋ effector memory T cells （TEM） in vitro and to improve the effect of adoptive immunotherapy. METHODS ： Normal human peripheral blood CD4＋ T-lymphocytes were isolated and randomly divided into 2 groups： control group and experiment group. VC was added into experimental group. Thereafter, the expansion of CD4＋ TEM in the 2 groups was detected by cell counter and flow cytometry. RESULTS： VC did not significantly affect the total number of CD4＋ T cells, while it raised the ratio of TEM in CD4＋ T cells at an optimal concentration of 100 mg/L. After 10 days of the expansion of CD4 ＋ T cells, the number of CD4＋ TEM in experiment group was （3.56 ±0.35 ）×10^6, significantly larger than that in control group [ （ 1.22 ± 0.15 ） ×10^6, P 〈 0.01 ]. CONCLUSION： VC can effectively promote the expansion of CD4＋ TEM in vitro, which provides a simple, safe and effective expansion method for adoptive immunotherapy. [
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2013年第8期1471-1475,共5页
Chinese Journal of Pathophysiology
基金
“十二五”艾滋病和病毒性肝炎等重大传染病防治科技重大专项基金资助项目(No.2012ZX10001-003-004
No.2013ZX10001-004)
国家自然科学基金资助项目(No.30972620
No.81101255)
广东省自然科学基金资助项目(No.9251008901000022
No.10451008901004204)
高等学校博士学科点专项科研基金资助项目(No.20090171110083
No.20100171120056)
