摘要
目的:观察N-Myc下游调节基因-2(N-Myc downstream-regulated gene 2,NDRG2)过表达对人膀胱癌T24细胞体外侵袭和迁移的影响。方法:采用携带NDRG2基因的慢病毒Lenti-NDRG2感染T24细胞,构建稳定过表达NDRG2的细胞株。Western blotting检测T24细胞中NDRG2、基质金属蛋白酶(matrix metalloproteinase,MMP)-2和MMP-9的表达,Transwell体外迁移和侵袭实验观察过表达NDRG2对T24细胞体外迁移和侵袭的影响。结果:建立了稳定过表达NDRG2的T24细胞株。与Lenti-Lacz组和空白对照组相比,Lenti-NDRG2组NDRG2蛋白的表达[(30.1±1.27)vs(9.9±1.24)、(8.2±1.28),P<0.01]明显提高;Lenti-NDRG2组MMP-2[(13.5±1.32)vs(30.7±1.29)、(28.8±1.30),均P<0.01]和MMP-9[(11.7±1.27)vs(25.2±1.28)、(26.4±1.31),均P<0.01]的表达明显降低;Lenti-NDRG2组T24细胞的侵袭细胞数[(18.1±3.4)vs(88.5±4.2)、(90.2±4.1)个,均P<0.01]和迁移细胞数[(20.1±3.5)vs(109.4±5.6)、(113.0±4.9)个,均P<0.01]明显减少。结论:慢病毒介导的NDRG2基因过表达可能通过降低MMP-2和MMP-9的表达而抑制人膀胱癌T24细胞的转移与侵袭。
Objective： To observe the effect of NDRG2 （ N-myc downstream-regulated gene 2） overexpression on inva- sion and metastasis of human bladder cancer 324 cell in vitro. Methods： The 324 cells were infected with lentivirus carry- ing NDRG2 gene （Lenti-NDRG2） to construct a stable cell line overexpression NDRG2. Western blotting was performed to examine the expression of NDRG2, matrix metalloproteinase （MMP） -2 and MMP-9 in 324 cells. The effects of NDRG2 overexpression on the invasion and migration of T24 cells were examined by the transwell invasion and migration assays, respectively. Results： The 324 cell line stable overexpression NDRG2 was successfully constructed. Compared with the LEN-LacZ group and the control group, the NDRG2 expression was efficiently increased in the Lenti-NDRG2 group （[30.1±1.27] vs [9.9±1.24], [8.2±1.28]; P〈0.01）; the MMP-2 （[13.5 ±1.32] vs [30.7±1.29], [28.8＋1.30]; P〈0.01） andMMP-9 expressions （[11.7±1.27] vs [25.2±1.28], [26.4±1.31]; P〈0.01） in T24 ceils of Lenti-NDRG2 group were significantly suppressed; the capabilities of the invasion （ [ 18.1± 3.4] vs [ 88.5 ± 4.2], [90.2 ±4.1] ; P〈0.01）and migration （[20.1 ±3.5] vs [109.4 ±5.6], [113.0 ±4.91 ; P 〈0.01） of P〈24 cells were significantly suppressed in the Lenti-NDRG2 group. Conclusion： The lentivirus-mediated NDRG2 overexpres- sion can suppress the invasion and migration of human bladder cancer T24 cells.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2013年第4期414-418,共5页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助项目(No.31070681)~~
