摘要
目的探讨野生型ｐ５３基因及其表达产物ｐ５３蛋白在恶性肿瘤基因治疗中的作用。方法应用腺病毒为载体将野生型ｐ５３基因转入高、低转移的肺癌细胞系Ａｎｉｐ９７３、ＡＧＺＹ８３－ａ，并且对各组转染细胞进行生长曲线、原位末端标记、Ｗｅｓｔｅｒｎ－ｂｌｏｔｔｉｎｇ等技术检测分析。结果野生型ｐ５３蛋白的过表达对上述肺腺癌细胞系均呈现出较强的生长抑制作用，野生型ｐ５３蛋白的过表达对高转移肺癌细胞系Ａｎｉｐ９７３的抑制作用明显高于低转移细胞系ＡＧＺＹ８３－ａ。结论外源性野生型ｐ５３基因的过表达，可以有效抑制上述肿瘤细胞的增殖，同时对高转移肺腺癌细胞系的抑制作用更加显著。
Objective To investigate the suppression effect of tumor suppressor gene p53 gene in lung adenocarcinoma cell lines.Methods Wild type p53 gene was transferred with adenovirus vector to a pair of lung adenocarcinoma cell lines with different metastasis potential: Anip973 (high - metastasis potential cell line) and AGZY83-a (low-metastasis potential cell line). The effects of p53 gene infection were evaluated by cell growth curve, western-blotting analysis and TUNEL technique. Results Overexpression of wild-type p53 gene in AGZY83-a, Anip973 inhibited the growth of both lung cancer cells and killed the cells in the end. The suppression effect of wild type p53 gene in Anip973 was higher than AGZY83-a while overexpression of p53 in this pair of cell line. Conclusion Wild-type p53 gene might act as a candidate gene in lung adenocarcinoma gene therapy.
出处
《哈尔滨医科大学学报》
CAS
2000年第4期240-242,F003,共4页
Journal of Harbin Medical University
基金
黑龙江省青年科学基金资助项目!(Q98-9)
黑龙江省科学技术计划攻关项目!(G99C20-6-1)
