摘要
本研究采用PCR-SSCP方法对陕北白绒山羊、太行黑山羊及南疆绒山羊的角蛋白辅助蛋白(keratin-associated pro-teins,KAP)7基因进行遗传多态性研究。结果显示,KAP7基因在3个山羊品种中有多态性,有AA和AB 2种基因型,AA基因型均为其优势基因型。测序结果发现,KAP7基因在129bp处存在C→G的突变,突变的位置位于5′调控区。
The polymerase chain reaction-single strand conformation polymorphism （PCR-SSCP） was used to detect the polymorphism of keratin associated protein 7 （KAP7） in Shanbei White cashmere goat,Taihang Black goat and Nanjiang White cashmere goat. The results showed that the KAP7 presented AA and AB genetypes in these three breeds,AA genetype was the dominant genetypes. The PCR production of different genotype of KAP7 were purified and sequnenced. One mutation, 129 bp（C→G） was found in KAPT. This mutation site was in 5 regulatory rezion.
出处
《中国畜牧兽医》
CAS
北大核心
2013年第7期133-136,共4页
China Animal Husbandry & Veterinary Medicine
基金
转基因生物新品种培育科技重大专项"高产绒量转基因绒山羊新品种培育"(2011ZX08008-002)
现代农业产业技术体系(CARS-40-13)
国家自然基金(31101684)
西北农林科技大学"拔尖人才支持计划"
江苏省高校"青蓝工程"
