摘要
目的 :探讨双黄连对rIFN α2a抗柯萨奇病毒的影响。方法 :采用微量细胞病变抑制法在Wish和Vero细胞上进行双黄连、rIFN α2a及两者联用抗柯萨奇病毒 (CVB3 )的实验研究。结果 :两药联用抗柯萨奇病毒呈明显协同作用 ;0 .12 5mg·ml-1的双黄连可使干扰素 (rIFN α2a)在Wish和Vero细胞上抗柯萨奇病毒的效价提高 2 .5 8倍左右。结论 :该结果为临床中西医结合治疗柯萨奇病毒感染提供新的配伍。
Objective :To investigate the effect of combined treatment of Shanghanglian (SHHL) and recombinant interferon α2a on Coxsackie virus B 3 replication. Methods: Microdose cytopathogenic inhibition effect assay was used in Wish and Vero cultured cells. rIFN α2a was applied 12 hours before Coxsackievirus B 3 inoculation and SHHL was applied 1 hour after Coxsackievirus B 3 inoculation.Results: 0.125 mg·ml -1 SHHL has a synergism with rIFN α2a to inhibit CPE caused by Coxsackievirus B 3 on the Wish and Vero cells . The anti coxsackievirus activity of combined treatment was 2.58 times higher than that of rIFN α2a treatment. Conclusion: This combined treatment of drugs may be useful in preventing and treating Coxsackie B 3 Virus infection. [
出处
《中国中药杂志》
CAS
CSCD
北大核心
2000年第11期682-684,共3页
China Journal of Chinese Materia Medica
