摘要
目的 :观察清开灵对大鼠谷氨酸神经毒性脑损伤的作用 ,以寻找有效的抗谷氨酸神经毒性的中西药物。方法 :采用细胞形态计量、电镜及免疫组织化学方法 ,观察清开灵对谷氨酸所致大鼠脑损伤的影响。结果 :清开灵可对抗谷氨酸所致的大鼠脑组织水、钠含量的增加 ,减轻谷氨酸所致的皮层及海马细胞的形态结构的损伤 ,降低脑组织中c fos阳性细胞率。结论 :清开灵具有抗谷氨酸神经毒性作用 ,其作用机制与抑制谷氨酸所致的c
Objective: To observe the effect of Qingkailing (QKL) on glutamate induced damage of brain, in order to seek for effective drugs for antagonizing neurotoxicity of glutamate. Methods: Number and morphology metrology of neuron in cerebral cortex and hippocampus were detected by using MIAS 300 image analysing instrument, electron microscope and immunohistochemical methods. Results: QKL could alleviate the glutamate induced accumulation of water and sodium in the brain tissue, relief the damage of cerebral cells and reduce the percentage of c fos positive cell in brain. Conclusion: QKL could protect brain damage induced by glutamate, which might be related to inhibition of QKL on glutamate caused enhancement of c fos gene expression.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2000年第11期842-845,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金资助课题!(No .3970 0 1 93)
关键词
谷氨酸
清开灵
实验研究
神经毒性脑损伤
cerebral damage, glutamate, Qingkailing, electron microscope, c-fos gene, cell morphological metrology
