摘要
目的观察贝母辛对肝纤维化大鼠肝组织α-SMA表达的影响,探讨贝母辛抗肝纤维化的作用机制。方法采用四氯化碳腹腔注射方法制作大鼠肝纤维化模型,设立正常对照组、肝纤维化模型组,贝母辛低、中、高剂量(2.5、5、10 mg/kg)组,疗程结束后,腹主动脉采血,检测血清ALT、AST、ALP、GGT表达。处死大鼠,取大鼠肝脏组织,Real time RT-PCR方法检测大鼠肝脏α-SMA mRNA表达水平;免疫组织化学检测α-SMA蛋白表达水平。结果模型组大鼠血清ALT、AST、ALP及GGT活性均显著高于正常对照组(P<0.01)。与模型对照组比,贝母辛低、中、高剂量组ALT、AST、ALP、GGT活性降低(P均<0.05)。Real time RT-PCR、免疫组化结果显示模型组α-SMA表达高于正常组,贝母辛中、高剂量组较模型组表达减少(P均<0.01)。结论贝母辛可有效抑制肝纤维化进展,其机制可能为干预肝星状细胞活化。
Objective： To observe the effect of peimisine on the expression of α-SMA in hepatic fibrosis tissues in rats.Methods： Hepatic fibrosis models induced by subcutaneous injection of CC14 in rats were randomly divided into the normal group,the model group and the peimisine groups including the low,the medium and the high dose of peimisine group.Levels were detected in the serum including the alanineaminotransferase（ALT）,the aspartate aminotransferase（AST）,the Alkaline Phosphatase（ALP） and the glutamyl transpeptidasecc（GGT）.The expressions of α-SMA in hepatic tissues were detected with Real time RT-PCR and immunohistochemical methods.Results： These indicatiors in the model group were higher significantly than those in the normal group such as ALT,AST,ALP,GGT（P 0.05）.However,above levels in the peimisine groups were lower obviouly than those in the model group（P 0.05）.From the Real time RT-PCR and immunohistochemical methods,the α-SMA expression in the model group was higher than that in the normal group,but the α-SMA expression in the peimisine groups such as medium and high dose were lower than those in the model group（all P 0.01）.Conclusion： Peimisine may prevent the formation of hepatic fibrosis by inhibition of the activation in hepatic stellate cell or promoting its apoptosis.
出处
《中国中医急症》
2013年第8期1274-1276,共3页
Journal of Emergency in Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81001573)
上海市第十人民医院"5810"培养计划
国家中医药管理局颜乾麟全国名老中医药专家传承工作室资助项目
