摘要
目的 检测脑缺血预处理大鼠脑组织中凋亡调控基因Bcl 2、Bcl xl和Bax的表达。方法 假手术对照组、预处理对照组、预处理缺血组和缺血组Wistar大鼠分别再灌 2 4h ,48h和 72h ,采用半定量逆转录聚合酶链反应 (RT PCR)和原位分子杂交 (ISH)方法对脑组织Bcl 2、Bcl xl和BaxmRNA的表达进行检测。结果 半定量RT PCR显示 :与假手术对照组相比 ,预处理对照组大鼠再灌 2 4h、48h和预处理缺血组大鼠再灌 2 4h脑组织Bcl 2mRNA的表达增高 ;预处理对照组和预处理缺血组大鼠再灌 2 4h脑组织Bcl xlmRNA的表达增高 (P <0 0 5 )。而四组大鼠BaxmRNA的表达无显著性差异 (P >0 0 5 )。ISH方法显示与假手术对照组及缺血组相比 ,预处理对照组和预处理缺血组大鼠再灌 2 4h脑组织Bcl 2、Bcl xlmRNA的表达增高 (P <0 0 5 ) ,各组大鼠BaxmRNA的表达无显著性差异 (P >0 0 5 )。结论 脑缺血预处理后Bcl 2、Bcl xlmRNA的表达增高 ,可能是脑缺血预处理内源性抗损伤的机制之一。
Objective To determine the expressions of apoptosis-regulated genes Bcl-2,Bcl-xl and Bax in rat cerebral ischemic preconditioning.Methods Forty-eight Wistar rats were randomly divided into four groups:sham-operation control group(SHC group),cerebral ischemic preconditioning control group(CIPC group),ischemic preconditioning-cerebral ischemia group(IPCI group)and cerebral ischemic group(CI group).The rats were reperfused in 24h,48h or 72h in each group following ischemia.Semi-quantity RT-PCR and in situ hybridization were adopted to investigate Bcl-2,Bcl-xl and Bax mRNAs expressions.Results Using semi-quantity RT-PCR,compared with those in SHC group,Bcl-2 mRNA expression increased greatly after 24h and 48h reperfusions in CIPC group and after 24h reperfusion in IPCI,guoup(P<)005),and Bcl-xl mRNA expression rose obviously after 24h reperfusion in CIPC and IPCI groups,while Bax mRNA expression were not significantly different among four groups(WTBX]P>005)P>005).Using in situ hybridization,Bcl-2 and Bcl-xl mRNA expressions increased after 24h reperfusion in CIPC and IPCI group(P<005), without significant difference in Bax mRNA expression among four groups (P>005).Conclusions The expressions of Bcl-2 and Bcl-xl mRNAs increase following cerebral ischemic preconditioning,suggesting that anti-apoptosis genes play important roles in the maintenance of cellular viability after ischemia.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2000年第9期540-542,共3页
Chinese Journal of Anesthesiology
