摘要
目的 了解上海医科大学华山医院大肠杆菌和肺炎克雷伯菌产超广谱 β 内酰胺酶(ESBLs)菌的发生率及其与细菌耐药性的关系。方法 双纸片法、酶抑制剂增强纸片扩散法、酶抑制剂联合三代头孢菌素最低抑菌浓度 (MIC)测定法和E 试验 ;Kirby Bauer(KB)法测定ESBLs株对抗菌药物的敏感性。结果 大肠杆菌中产ESBLs菌株占 2 5 % ,肺炎克雷伯菌中为 5 7% ;上述产ESBLs株对第三代头孢菌素和氨曲南大多耐药 ,亚胺培南的耐药株少见 ,对头孢西丁的敏感率近 80 % ,对阿米卡星、环丙沙星等大多呈现耐药。结论 超广谱 β 内酰胺酶是细菌对第三代头孢菌素、单环 β 内酰胺类抗生素耐药的重要机制 。
Objective To investigate the incidence of extended spectrum β lactamases (ESBLs) of E.coli and K. pneumoniae in Hua Shan Hospital of Shanghai Medical University and their relationship with antibiotic resistance patterns.Methods ESBLs producing strains in E.coli and K. pneumoniae were detected by double disc synergy test, inhibitor potentiated disc diffusion test and inhibitor combined with minimum inhibitory concentrations (MIC) determination of third generation cephalosporins. The bacterial susceptibility testing of ESBLs producing strains was assayed by Kirby Bauer method.Results The incidence of ESBLs was 25% in E.coli and 57% in K. pneumoniae. ESBLs producing strains were more resistant to third generation cephalosporins and aztreonam, but few of which was resistant to imipenem. The susceptibility rate of these strains to cefoxitin was about 80%. Most strains were resistant to amikacin and ciprofloxacin.Conclusion Extended spectrun β lactamases play a most important role in bacterial resistance to third generation cephalosprins and monobactams. We should pay much attention to detecting ESBLs producing strains and their susceptibility to antibiotics.
出处
《中华传染病杂志》
CAS
CSCD
北大核心
2000年第3期151-154,共4页
Chinese Journal of Infectious Diseases
关键词
超广谱Β-内酰胺酶
细菌耐药性
药敏试验
Extended spectrum β lactamases
Escherichia coli
Klebsiella pneumoniae
