摘要
目的:建立测定益肾蠲痹丸中淫羊藿苷含量的方法。方法:采用高效液相色谱法。色谱柱为KromasilC18(250mm×4.6mm,5μm),流动相为乙腈-水(30∶70,V/V),流速为1.0ml/min,柱温为35℃,检测波长为270nm。结果:淫羊藿苷的进样量在0.0813～1.2192μg范围内与其峰面积积分值呈良好的线性关系(r=0.9995);精密度、稳定性、重复性试验的RSD均<2%;平均加样回收率为100.66%,RSD=1.92%(n=9)。结论:该方法简便、准确、重复性好,可有效控制益肾蠲痹丸的质量。
OBJECTIVE： To establish a method for the content determination of icariin in Yishen juanbi pills. METHODS： HPLC method was adopted. The determination was performed on Kromasil C18（250 mm×.6 mm, 5 μ） column with mobile phase consisted of acetonitrile-water （30 ： 70, V/V） at the flow rate of 1.0 ml/min. The column temperature was 35 ℃ and the detection wavelength was set at 270 nm. RESULTS： The linear range of icariin were 0.081 3-1.219 2μg（r=0.999 5） with an average recov- ery of 100.66% （RSD=0.92%, n=9）. The RSDs of precision,reproducibility and stability tests were lower than 2% .CONCLU- SIONS： The method is simnle, accurate and renroducible, and it can be used for the aualitv control of Yishen iuanbi oills.
出处
《中国药房》
CAS
CSCD
2013年第31期2949-2950,共2页
China Pharmacy
