摘要
为筛选和验证人工锌指核酸酶的活性,本研究基于SSA原理,采用LA-PCR法和基因工程操作技术构建了1个荧光素酶基因报告载体。经菌落PCR、限制性内切酶鉴定及DNA测序验证表明,锌指核酸酶筛选验证的报告载体构建成功,为进一步开展锌指核酸酶技术研究提供了试验平台。
To screen and identify the activity of zinc finger nuclease （ZFN） ,according to SSA prin-ciple,the luciferase gene reporter system was constructed by long and accurate PCR （LA-PCR） and gene engineering technology in the present study. The ZFN reporter vector was successfully constructed and confirmed by PCR using colonies, restriction endonuclease digestion analysis and sequencing that become a test bed for further experiments.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第8期1263-1266,共4页
Chinese Journal of Veterinary Science
基金
桂渔牧科资助项目(1204917)
桂科自资助项目(2010GXNSFA013096)
广西‘特聘专家’专项经费资助项目
桂科攻资助项目(1123005-1)
科技部国际科技交流与合作专项资助项目(2008DFA30320)
农业部公益性行业科研专项资助项目(SQ201303118)
关键词
SSA
锌指核酸酶
荧光素酶基因
single strand annealing
zinc finger nuclease
luci{erase gene
